African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2199

Full Length Research Paper

The role of nitric oxide in alendronate-mediated acceleration of gastric emptying and gastrointestinal transit in rats

Renan Oliveira Silva
  • Renan Oliveira Silva
  • Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceara, Fortaleza, CE, Brazil.
  • Google Scholar
Rudy Diavila Bingana
  • Rudy Diavila Bingana
  • Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceara, Fortaleza, CE, Brazil.
  • Google Scholar
Pedro Marcos Gomes Soares
  • Pedro Marcos Gomes Soares
  • Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceara, Fortaleza, CE, Brazil.
  • Google Scholar
Marcellus Henrique Loiola Ponte Souza
  • Marcellus Henrique Loiola Ponte Souza
  • Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceara, Fortaleza, CE, Brazil.
  • Google Scholar
Jand Venes Rolim Medeiros*
  • Jand Venes Rolim Medeiros*
  • Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piaui, Parnaiba, PI, Brazil.
  • Google Scholar


  •  Received: 03 March 2015
  •  Accepted: 11 March 2015
  •  Published: 15 March 2015

Abstract

The effect of the alendronate (ALD; 30 mg/kg, pH 7.0, p.o.) and sodium nitroprusside (SNP; 10 mg/kg, p.o.; a nitric oxide (NO) donor) on gastric dye retention (GDR) and proximal, medial and distal small intestine dye retentions (IDR) was investigated in rats. The drugs were administered once daily for 4 days. On the last day of treatment, 4 h after ALD administration, GDR and IDR were measured. ALD treatment decreased GDR at postprandial intervals of 20 (28.5%) and 30 min (38.3%), while it increased medial IDR (117.2%), as compared to the saline group. ALD had no effect on dye retention in proximal and distal portions of the small intestine. In 30 min, ALD increased medial (50.5%) and distal IDR (149.7%), as compared to the saline group. Pretreatment with SNP prevented ALD from decreasing gastric retention and intestinal transit. The results of this study indicated that ALD accelerates gastric emptying of liquids in rats and support the hypothesis that the inhibition of nitric oxide is of primary importance.

Key words: Gastric emptying, gaseous mediators, nitric oxide, alendronate.