African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2162

Full Length Research Paper

Investigation of quercetin stability in cell culture medium: Role in in vitro experiment

Jun Hu1, Long Chen1, Fan Lei1, Yu Tian1,2, Dong-Ming Xing1, Yu-Shuang Chai1, Shuang Zhao1, Yi Ding1,2 and Li-Jun Du1*
1Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China. 1Drug Discovery Facility, Tsinghua University, Beijing 100084, China.
Email: [email protected]

  •  Accepted: 23 March 2012
  •  Published: 15 April 2012

Abstract

The aims of this work are to investigate the in vitro stability of quercetin, a focused compound in food components and a candidate drug, and to supply the essential information for the experimental researches of quercetin. The stability of quercetin in Dulbecco’s modified Eagle’s medium (DMEM) and H2O at the cell culture pH range of 6 of 8 at continued time points, as well as 6 frequently used supplements in DMEM was investigated. The assay of high-performance liquid chromatography (HPLC) was used for quercetin determination. Considering the stable conditions of quercetin, the transport behavior of quercetin in Neuro-2a (N2a) cells was investigated at the dosages of 58.8 and 7.83 μg/ml at 37°C for different time points. It is shown that quercetin remained stable in DMEM at pH of 6 with or without 10 mM/L HEPES, 5% D (+)-glucose, 110 mg/L sodium pyruvate, antibiotics (50 IU/ml penicillin and 0.05 mg/ml streptomycin), or 10% fetal bovine serum (FBS), but was decreasing at pH of 7 or 8 with or without 3.7 g/L NaHCO3. Quercetin transported through N2a cells in 10 min in both dosages of 58.8 and 7.83 μg/ml. Our data demonstrate that the culture conditions of quercetin in vitroshould be considered to acquire the convictive results in the experiments.

 

Key words: Quercetin, stability, high performance liquid chromatography (HPLC), in vitro, transportation.