African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 718

Full Length Research Paper

A comparative study of antimicrobial and antioxidant activities of garlic (Allium sativum L.) extracts in various localities of Pakistan

Muhammad Gulfraz
  • Muhammad Gulfraz
  • Institute of Biochemistry and Biotechnoogy, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
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Muammad Imran*
  • Muammad Imran*
  • Institute of Biochemistry and Biotechnoogy, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
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Sobia Khadam
  • Sobia Khadam
  • Institute of Biochemistry and Biotechnoogy, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
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Dawood Ahmed
  • Dawood Ahmed
  • Institute of Biochemistry and Biotechnoogy, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
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Muhammad J. Asad
  • Muhammad J. Asad
  • Institute of Biochemistry and Biotechnoogy, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
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Kashis S. Abassi
  • Kashis S. Abassi
  • Institute of Biochemistry and Biotechnoogy, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
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Muhammad Irfan
  • Muhammad Irfan
  • Institute of Biochemistry and Biotechnoogy, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
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Sajid Mehmood
  • Sajid Mehmood
  • Institute of Biochemistry and Biotechnoogy, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
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  •  Received: 10 September 2011
  •  Accepted: 07 May 2014
  •  Published: 30 June 2014

Abstract

The present study was conducted to analyze garlic extracts using compositional analysis and assessment of antibacterial and antioxidant properties against various microbes. It was observed that garlic consist of various bioactive compounds such as alkaloids, phenols, tannins and flavonoids with different concentration levels. Antimicrobial activity of different solvent extracts (n-hexane, chloroform, acetone, butanol and methanol) was tested against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus epidermidis and Klebsella pneumonia by using agar well diffusion assay. Results indicate that all the extracts were effective against the microorganism tested but n-hexane extract showed minimum activity against all microbes. Methanol extract was proven to be more effective against the K. pneumonia. MIC of these extracts was checked from 1-15 mg/mg concentration levels. Garlic plant extracts have rich contents of alkaloids, flavonoids, tannins and phenols. Various bioactive compounds are responsible for its medicinal properties.

 

Key words: Antimicrobial activity, antioxidant activity, compositional analysis, flavonoids, alkaloids.