African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 688

Full Length Research Paper

Phytochemical analysis of Phyllanthus niruri L. (Phyllanthaceae) extracts collected in four geographical areas in the Democratic Republic of the Congo

Rufin Kikakedimau Nakweti1,2*, Sébastien Luyindula Ndiku1*, Patrick Doumas3, Mutambel’ Hity Schie Nkung4, Yves Baissac6, Richard Cimanga Kanyanga5, Aimé Diamuini Ndofunsu1, Freddy Bulubulu Otono1 and Christian Jay-Allemand6
  1Department of Biotechnology and Molecular Biology, Division of Life Sciences, General Atomic Energy Commission, Regional Center of Nuclear Studies of Kinshasa (GAEC/RCNS-K.), BP 868 Kinshasa XI, P.O.BOX 868 KIN.XI, Democratic Republic of the Congo (DRC). 2Plant Physiology and Molecular, PHD Student, Department of Biology, Faculty of Sciences, University of Kinshasa, P.O.BOX 190 KIN.XI, DRC. 3Biochimie et Physiologie Moléculaire des plantes, UMR 5004 (Agro-M/CNRS/INRA/UM2), Montpellier SupAgro; Montpellier/France. 4Department of Microbiology, General Atomic Energy Commission. Regional Center of Nuclear Studies of Kinshasa .P.O.BOX 868 KIN.XI, DRC. 5Faculty of Pharmacy, University of Kinshasa, P.O.BOX 834, Kinshasa XI, DRC. 6Laboratory of Plant Physiology and Biochemistry at the University of Montpellier II (UM2), Montpellier/France.
Email: [email protected], [email protected]

  •  Accepted: 13 June 2012
  •  Published: 31 January 2013

Abstract

 

This study aims at investigating the phytochemical analysis or to analyze the secondary metaboliotes of Phyllanthus niruri L. plants from four collection sites which University of Kinshasa (Unikin), National Pedagogic University of Kinshasa (UPN), Kimwenza (Kim) and Kisantu (Kis) in the Democratic Republic of the Congo (DRC). This study should give an explanation about the change of antiplasmodial activity of the same plant depending on the location of harvest. The samples of P. niruri were analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) for secondary metabolites characterizations such as of flavonoids, saponins and steroidal sapogenins and others phenolic compounds. The results revealed that UPN location presented more peaks (22) than Unikin (20), Kimwenza (15) and Kisantu (12). But Unikin location revealed more peaks (7) corresponding to major compounds than samples from others locations (UPN: 5; Kimwenza: 2 and Kisantu: 4). The peak 1 of UPN is higher (13.73) comparing to all peaks samples. The yellow-colored spots were present at all samples but those of UPN were more accentuated than all. In Kimwenza samples, two others colored spots (violet and blue) were presented. It suggested that in vitroantiplasmodial activity would be based on compounds eluted probably at the retention time around 22 min. Sometime the compounds eluted at 4.28 and 7.8 min contribute to in vitro antiplasmodial activity. The results revealed again the presence of the saponins or the steroidal sapogenins in P. niruri, made for the characterization by HPLC or by TLC probables flavonoids and presence of steroidal sapogenins. 

 

Key words: Thin layer chromatography (TLC), high performance liquid chromatography (HPLC), Phyllanthus niruri, callus.

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