African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 728

Full Length Research Paper

Effect of auxins and auxin polar transport inhibitor (TIBA) on somatic embryogenesis in groundnut (Arachis hypogaea L.)

K. Venkatesh1, A. Roja Rani2*, Nirmala Baburao2 and G. Padmaja3
  1Department of Genetics, Osmania University, Hyderabad 500 007, India. 2Department of Botany, Osmania University College for Women, Hyderabad 500095, India. 3School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India.
Email: [email protected]

  •  Accepted: 28 October 2009
  •  Published: 31 December 2009

Abstract

 

The phytohormone auxin plays an important role in growth, developmental and physiological processes. The effect of auxins, 2,4-D, NAA, IAA, Dicamba and picloram, was tested for somatic embryogenesis in groundnut. Among the different auxins tested 2,4-D favored the best response of somatic embryogenesis with induction of 18.3 somatic embryos per explant that were big, healthy, succulent and green in color. Immature zygotic embryos axes cultured on MS medium with 4 mg/l TIBA (Auxin polar transport inhibitor) did not respond for somatic embryogenesis but when cultured on medium supplemented with TIBA (2 mg/l and 4 mg/l) and 2,4-D (4 mg/l) showed the induction of somatic embryogenesis. When the concentration of TIBA was increased (6 mg/l) keeping the level of 2, 4-D (4 mg/l l) constant, it resulted in browning of explants within a week of culture. These observations demonstrate the requirement of 2,4-D for induction of somatic embryogenesis and that addition of TIBA (2 mg/l and 4 mg/l) decreases the response of somatic embryogenesis and further higher levels (6 mg/l) being inhibitory for somatic embryogenesis.

 

Key words: Auxin polar transport inhibitor, somatic embryogenesis.