Genomic DNA was prepared from germinating seeds of a variety of sorghum in Nigeria (Samsorg 41) using two different isolation protocols (CTAB and morning fresh® method). Highest quantity of DNA was obtained from Samsorg 41 using the morning fresh® method. Genomic DNA obtained using this method had a much lower purity as compared with that obtained using the CTAB method. Both methods produced genomic DNA of high integrity with minimum shearing.
Key words: Genomic DNA, CTAB, morning fresh®, isolation, Samsorg 41, Nigeria.
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