African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 686

Full Length Research Paper

Comparative analysis of genomic DNA isolation procedures; hexa decyltrimethylammonium bromide (CTAB) and liquid detergent (morning fresh®) methods from Samsorg 41 (icsv 400)

Inuwa H. M.1, Aimola I. A.2* and Inuwa L.1
1Biochemistry Department, Ahmadu Bello University, Zaria, Nigeria. 2Department of Biochemistry, National Research Institute for chemical Technology, Zaria, Nigeria.
Email: [email protected]

  • Article Number - 4F1BF0D9002
  • Vol.5(4), pp. 222-225, April 2011
  •  Accepted: 22 February 2011
  •  Published: 30 April 2011

Abstract

Genomic DNA was prepared from germinating seeds of a variety of sorghum in Nigeria (Samsorg 41) using two different isolation protocols (CTAB and morning fresh® method). Highest quantity of DNA was obtained from Samsorg 41 using the morning fresh® method. Genomic DNA obtained using this method had a much lower purity as compared with that obtained using the CTAB method. Both methods produced genomic DNA of high integrity with minimum shearing.

 

Key words: Genomic DNA, CTAB, morning fresh®, isolation, Samsorg 41, Nigeria.

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