African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 686

Full Length Research Paper

Production of exopolygalacturonase from wheat flour by Aspergillus awamori in submerged and surface culture fermentation

Heidar Abbasi* and Seyyed Ruhollah Mortazavipur
University of Applied Science and Technology of Khormuj, Bushehr, Iran.
Email: [email protected]

  • Article Number - 74071D98996
  • Vol.5(4), pp. 226-232 , April 2011
  •  Accepted: 14 March 2011
  •  Published: 30 April 2011

Abstract

Pectinases are a group of hydrolytic enzymes that degrade pectic substances. They are produced by a variety of microorganisms including filamentous fungi. Studies were carried out on the production of exoplygalacturonase (Exo-PGase) using wheat flour by Aspergillus awamori in submerged fermentation (SmF) and surface culture fermentation (SCF). Higher titres of was observed when medium was supplements with carbon (4 g/L Pectin for both SmF and SCF) and nitrogen (ammonium sulphate 6 g/L for both SmF and SCF) sources. SCF proved to be a better process than SmF for Exo-PGase production, giving the highest enzyme production rate in 96 h. Exo-PGase produced by A. awamori presented optimum activity at pH 5 for SmF and 5.5 for SCF. Use of continuous system for Exo-PGase production obtained a better results than the batch system.

 

Key words: Exopolygalacturonase, Aspergillus awamori, wheat flour, surface culture fermentation, submerged fermentation, continuous production.

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