African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 727

Full Length Research Paper

RAPD and SSR analysis of wild oats (Avena species) from North West frontier Province of Pakistan

Zahid Hanif1, Zahoor A. Swati1, Imtiaz Khan2*, Gul Hassan2, K. B. Marwat2, sabz Ali3 and M. Ishfaq Khan2
1Institute of Biotechnology and Genetic Engineering, NWFP, Agricultural University Peshawar-Pakistan. 2Department of weed science, NWFP Agricultural University Peshawar-Pakistan. 3Plant breeding group, Plant research international, Wageningen University, Netherlands.
Email: [email protected]

  •  Accepted: 26 November 2008
  •  Published: 30 November 2008

Abstract

Genetic diversity of 10 biotypes of wild oats (Avena species) which differ in certain morphological and physiological characters were analyzed using 9 Randomly Amplified Polymorphic DNA (RAPD) and 2 Simple Sequence Repeat (SSR) primer sets. RAPD and SSR primers revealed different levels of genetic polymorphisms among the various biotypes. Range of genetic distances estimated by using individual primers was 0 – 100 % but on an average genetic distances were in the range of 30 - 65% indicating that these biotypes were genetically very diverse and possess a high degree of polymorphism.

 

Key words: Wild oats, DNA, Polymorphism, RAPD, SSR.