African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 728

Full Length Research Paper

Characterization of callus formation in leaf of Euphorbia helioscopia

Zi-Song Yang1,3, Guang-Deng Chen2,3*, Yun-Xiang Li2,3 and Jiao Chen2,3
  1Department of Chemistry and Life Science, Aba Teachers College, Pixian, 611741, People’s Republic of China. 2College of Life Science, China West Normal University, Nanchong, 637009, People’s Republic of China. 3Key Laboratory of Southwest China Wildlife Resources Conservation (China West Normal University), Ministry of Education, Nanchong, 637009, People’s Republic of China.
Email: [email protected]

  •  Accepted: 11 June 2009
  •  Published: 30 June 2009



The callus was induced on Murashige and Skoog’s (MS) medium supplemented with 2,4-dichlo-rophenoxyacetic acid (2,4-D) or 6-Benzylaminopurine (BAP) from the Chinese medicinal herb Euphorbia helioscopia in the family Euphorbiaceae. The highest frequencies of callus induction were observed on MS medium supplemented with 3.0 mg l-1 2,4-D. Callus (yellow, loose, granular) was chosen as research focus in the long-term callus maintenance, the proliferation coefficient could still hold about 10 after subcultured 5 turns. Histological analysis was done to reveal the developmental pattern of callus formation. Detailed analysis revealed induction of leaf yielded many endogenous eumeristematic cells which were still embedded in the parenchymatic tissue.


Key words: Euphorbia helioscopia, callus induction, callus maintenance, histological, developmental pattern.