African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 790

Full Length Research Paper

Antibacterial activity of some substances against citrus bacterial canker caused by Xanthomonas citri pv. citri (Hasse) in Burkina Faso

ZERBO Kevin Ben Fabrice
  • ZERBO Kevin Ben Fabrice
  • Centre National de la Recherche Scientifique et Technologique/Institut de l’Environnement et de Recherches Agricoles (INERA)/Laboratoire Mixte International/Observatoire des Agents Pathogènes, Biosécurité et Biodiversité (LMI PathoBios), 01 BP 910 Bobo Dioulasso 01, Burkina Faso.
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WONNI Issa
  • WONNI Issa
  • Centre National de la Recherche Scientifique et Technologique/Institut de l’Environnement et de Recherches Agricoles (INERA)/Laboratoire Mixte International/Observatoire des Agents Pathogènes, Biosécurité et Biodiversité (LMI PathoBios), 01 BP 910 Bobo Dioulasso 01, Burkina Faso.
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YAMEOGO Florence
  • YAMEOGO Florence
  • Centre National de la Recherche Scientifique et Technologique/Institut de l’Environnement et de Recherches Agricoles (INERA)/Laboratoire Mixte International/Observatoire des Agents Pathogènes, Biosécurité et Biodiversité (LMI PathoBios), 01 BP 910 Bobo Dioulasso 01, Burkina Faso.
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SOMDA Irénée
  • SOMDA Irénée
  • Université Nazi BONI/Institut de Développement Rural/Clinique des Plantes; 01 BP1091, Bobo Dioulasso, Burkina Faso.
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  •  Received: 24 March 2022
  •  Accepted: 08 June 2022
  •  Published: 31 July 2022

Abstract

Citrus bacterial canker (CBC) threatens citrus fruit production in Burkina Faso due to the lack of effective control methods. In this study, the antimicrobial effect of copper hydroxide and sulphate, the biopesticide from Bacillus amyloliquefaciens, the essential oil of Ocimum gratissimum and aqueous extracts of Eucalyptus camadulensis, Azadirachta indica and Cymbopogon citratus were tested in vitro and in vivo against Xanthomonas cirri pv. citri (Hasse) (Xcc890) strain from Burkina Faso. The dilution method in solid medium and macro-method dilution in liquid medium were, respectively used in order to determine the minimum bactericidal concentration (MBC) and the minimum inhibitory concentration (MIC). Hydroxide and sulphate copper tested at 25 mg/ml induced 13 mm in diameter of inhibition zone, followed by B. amyloliquefaciens at 25 µl/ml (11.6±3.9 mm). O. gratissimum induced 10.5 ± 0.11 mm of inhibition zone at 5 µl/ml. Aqueous extract of E. camadulensis at 100 mg/ml induced 11.4 ± 1 mm of inhibition diameter. Based on MBC/MIC ratio, copper hydroxide and sulphate, O. gratissimum and E. camadulensis have a bactericidal effect in vitro. In vivo test, copper products at 25 mg/ml and O. gratissimum at 10 µl/ml were the most effective and significantly reduced the CBC incidence. These substances can be evaluated under natural conditions in order to determine their effectiveness against CBC.

 

Key words: Citrus species, citrus bacterial canker, Xanthomonas citri pv. citri, antibacterial activity, substances.