African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 718

Full Length Research Paper

Anticancer activity of n-hexane extract of Cichorium intybus on lymphoblastic leukemia cells (Jurkat cells)

Mohammad Saleem*
  • Mohammad Saleem*
  • University College of Pharmacy, University of the Punjab, Lahore, Punjab, Pakistan; College of Pharmacy, GC University, Faisalabad, Punjab, Pakistan.
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Khizar Abbas
  • Khizar Abbas
  • University College of Pharmacy, University of the Punjab, Lahore, Punjab, Pakistan.
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Faiza Naseer
  • Faiza Naseer
  • University College of Pharmacy, University of the Punjab, Lahore, Punjab, Pakistan.
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Mobasher Ahmad
  • Mobasher Ahmad
  • University College of Pharmacy, University of the Punjab, Lahore, Punjab, Pakistan.
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Nawazish-i-Husain Syed
  • Nawazish-i-Husain Syed
  • University College of Pharmacy, University of the Punjab, Lahore, Punjab, Pakistan.
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Fatima Javed
  • Fatima Javed
  • University College of Pharmacy, University of the Punjab, Lahore, Punjab, Pakistan.
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Khalid Hussain
  • Khalid Hussain
  • University College of Pharmacy, University of the Punjab, Lahore, Punjab, Pakistan.
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Samia Asima
  • Samia Asima
  • Pharmacy Department, The University of Lahore, Pakistan.
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  •  Received: 18 February 2013
  •  Accepted: 09 June 2014
  •  Published: 30 June 2014

Abstract

Cichorium intybus commonly known as chicory is a member of the family Compositeae. It has been known for various pharmacological activities and anticancer effects against various cell lines including breast cancer MCF-7, prostate cancer LNCaP, amelanotic melanoma C32 and renal adenocarcinoma ACHN. In the present study, aerial parts of C. intybus were collected, identified, soaked in n-hexane non polar solvent, filtered and evaporated till the extract residue was isolated, dried and stored at 4°C and then checked for cytotoxic activities. Lymphoblastic leukemia cells (Jurkat cells) were used to evaluate the cytotoxic effects of n-hexane extract of C. intybus. Extracts were added to the cultured cells of selected cell line in various concentrations (10, 25, 50, 75 and 100 µg/ml) and incubated for 24 h. Trypan blue exclusion assay, MTS assay and FACS analysis were carried out to analyze cell viability, cell proliferation and apoptosis, respectively. The non polar n-hexane extract significantly reduced the number of viable cells and cell proliferation percentage but induced the apoptosis haphazardly. Results of this study demonstrate that n-hexane extract of C. intybus has potent anti-proliferative and cytotoxic activity against Jurkat cells, a human leukemia cell line.

 

Key words: Cichorium intybus, Jurkat cells, lymphoblastic leukemia cells, MTS, trypan blue assay, apoptotic analysis.