African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 728

Full Length Research Paper

Molecular analysis of type III polyketide synthase (PKS) gene family from Zingiber officinale Rosc

Radhakrishnan E. K. and E. V. Soniya*
Plant Molecular Biology Division, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India
Email: [email protected]

  •  Accepted: 20 March 2009
  •  Published: 31 March 2009

Abstract

 

Enzymes of the type III polyketide synthase family is considered to have significant role in biosynthesis of structurally diverse polyketide scaffolds in Zingiber officinale. Genome wide analysis of polyketide synthase gene family in Z. officinale identified partial sequences of six members. Comparative sequence analysis showed that four of them ZoPKS2, 3, 4 and 6 were novel forms as revealed by the significant variations at the highly conserved regions. Phylogenetic analysis also showed separate clustering of the novel forms along with the non-chalcone forming PKSs. This sequence identified in the study forms the first and basic information of the PKS gene family from the Zingiberaceae.

 

Key words: Zingiber officinale, CHS, PKS, multigene family, phylogenetic analysis.