African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 809

Full Length Research Paper

In vitro protocol optimization for micropropagation of elite Lemmon verbena (Aloysia triphylla)

Mengistu Fentahun
  • Mengistu Fentahun
  • Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, Holeta, Ethiopia.
  • Google Scholar
Solomon Tamiru
  • Solomon Tamiru
  • Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, Holeta, Ethiopia.
  • Google Scholar
Tsion Tessema
  • Tsion Tessema
  • Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, Holeta, Ethiopia.
  • Google Scholar
Diriba Guta
  • Diriba Guta
  • Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, Holeta, Ethiopia.
  • Google Scholar
Helen G/Medhin
  • Helen G/Medhin
  • Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, Holeta, Ethiopia.
  • Google Scholar


  •  Received: 13 July 2017
  •  Accepted: 07 September 2017
  •  Published: 31 October 2017

References

Andargachew GA (2007). Indigenous Knowledge and Genetic Diversity of Cultivated Amochi (Arisaema schimperianum Schott), PhD thesis, Department of Environmental and Plant Sciences, Norwegian university of life sciences, Norway, pp. 1-49.

 

Ansar A, Touqeer A, Nadeem AA, Ishfaq AH (2009). Effect of different concentrations of auxins on in vitro rooting of olive cultivar 'Moraiolo'. Pak. J. Bot. 41(3):1223-1231.

 

Armada J, Barra A (1992). On Aloysia Palau (Verbenaceae). Taxon 41:88-90.
Crossref

 

Beemnet M, Omarsherif M, Tsion T, Solomon A (2010). Production, Processing and Utilization of Aromatic Plants, Ethiopian Institute of Agricultural Research (EIAR), Addis Ababa, Ethiopia, P 31.

 

Beemnet M, Wondu B, Solomon A (2013). Performance of Lemon Verbena (Aloysia triphylla L.) for Morphological, Economic and Chemical Traits in Ethiopia. Am. Eurasian J. Agric. Environ. Sci. 13(11):1576-1581.

 

Benelli C, Fabbri A, Grassi S, Lambardi M, Rugini E (2001). Histology of somatic embryogenesis in mature tissues of olive (Olea europaea L.). J. Hortic. Sci. Biotechnol. 76(1):112-119.
Crossref

 

Bilia AR, Giomi M, Innocenti M, Gallori S, Vincieri FF (2008). HPLC-DAD-ESI-MS analysis of the constituents of aqueous preparations of verbena and lemon verbena and evaluation of the antioxidant activity. J. Pharm. Biomed. Anal. 46:463-470.
Crossref

 

Botta S (1979). Las especies argentinas del genero Aloysia (Verbenaceae). Darwiniana 22:67-108.

 

Braga F, Mendes C, Raphael R, Oliveira T, Soares C, Resende F, Pinto C, Santana D, Viccini F, Raposo N, Peixoto P (2011). Micropropagation, antinociceptive and antioxidant activities of extracts of Verbena litoralis Kunth (Verbenaceae). Acad. Bras. Cienc. 84(1):139-147.
Crossref

 

Burdyn L, Luna C, Tarrago J, Sansberro P, Dudit N, Gonzalez A, Mroginski L (2006). Direct shoot regeneration from leaf and internode explants of Aloysia polystachya [gris.] mold. (Verbenaceae). In vitro Cell. Dev. Biol. Plant 42:235-239.
Crossref

 

Carnat A, Carnat AP, Fraisse D, Lamaison JL (1999). The aromatic and polyphenolic composition of lemon verbena tea. Fitoterapia 70:44-49.
Crossref

 

Carnat A, Carnat P, Fraisse D, Ricoux L, Lamaison JL (2004). The aromatic and polyphenolic composition of Roman camomile tea, Fitoterapia 75:32-38.
Crossref

 

Cowan MM (1999). Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12:564-582.

 

De Klerk G. (2002). Rooting of microcuttings: theory and practice. In Vitro Cell. Dev. Biol. Plant 38:415-422.
Crossref

 

Deepa VS, Rajaram K, Kumar MA, Das S, Kumar PS (2011). High frequency regeneration and shoot multiplication in Andrographis lineate wall. ex. nees: an endemic medicinal plant of south India. J. Med. Plant Res. 5(20):5044-5049.

 

Demsachew G (2011). Micropropagation of two apple (Malus domestica Borkh) varieties from shoot tip explants. An M.Sc Thesis presented to the school of Graduate Studies of Haramaya University, Haramaya, Ethiopia. P 60.

 

Durat T, Christina M (2005). Anti-Candida activity of Brazilian medicinal plants. J. Ethnopharmacol. 97(2):305-311.
Crossref

 

EIAR (Ethiopian Institute of Agricultural Research) (2009). MOU agreement between Ariti Helblal plc, Fana Aromatic and Medicinal plants growers' private limited association and Wondo Genet Agricultural Research Center for production, processing and commercialization of Aromatic and Medicinal Plants, Ethiopian Institute of Agricultural Research (EIAR), Addis Ababa, Ethiopia, P 10.

 

Gomes PCS, Oliveira HRC, Vicente AMS, Ferreira MF (2006). Production, transformation and essential oils composition of leaves and stems of lemon verbena [Aloysia triphylla (L'Herit.) Britton] grown in Portugal. Rev. Bras. Pl. Med. 8:130-135.

 

Graca JAB, Henriques JML, Medina G, Monteiro L, Oliveira LC, Pereira TG, Ramilo MT (1996). Guia prático de remédios e tratamentos naturais," Selecções do Reader's Digest, Lisboa, Portugal, P. 150.

 

Gupta S, Khanuja S, Kumar S (2001). In vitro micropropagation of Lippia alba. Curr. Sci. 81(2):206-210.

 

Hanna FMA, El-Beltagi HS, Nasr NF (2011). Evaluation of Antioxidant and Antimicrobial Activity of Aloysia triphylla. Electronic J. Environ. Agric. Food Chem. 10(8):2689-2699.

 

Juliani Jr H, Koroch A, Juliani H, Trippi V (1999). Micropropagation of Lippia junelliana (Mold.) Tronc. Plant Cell Tissue Organ Cult. 59:175-179.
Crossref

 

Krieken WVD, Breteler H, Visser MHM, Mavridou D (1993). The role of the conversion of IBA into IAA on root regeneration in apple. Introduction of test system. Plant Cell Rep. 12:203-206.
Crossref

 

Mosavi AA (2012). The Optimization of Lemon Verbena (Lippia citriodora) Medicinal Plant Tissue Culture. Int. J. Agron. Plant. Prod. 3(11):561-565.

 

Murashige T, Skoog F (1962). A revised media for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497.
Crossref

 

Naghmouchi S, Khouja ML, Rejeb MN, Boussaid M (2008). Effect of growth regulators and explant origin on in vitro propagation of Ceratonia siliqua L. via cuttings. Biotechnol. Agron. Soc. Environ. 12(3):251-258.

 

Newal CA, Anderson LA, Phillipson JD (1996). Herbal medicines. A guide for health-care professionals, the pharmaceutical press, London, U.K., P 179.

 

NMSA (National Metrology Service Agency) (1996). Climatic and Agro climatic Resources of Ethiopia, National Metrology Service Agency of Ethiopia, Addis Ababa, 1:137.

 

Oladzad A, Qaderi A, Naghdi Badi H, Zare A (2012). Rapid Micropropagation of Lemon Verbena (Lippia citriodora L.) Using In vitro Culture. J. Med. Plants 2(42):145-153.

 

Peixoto P, Salimena F, Santos M, Garcia L, Pierre P, Viccini L, Otoni W. (2006). In vitro propagation of endangered Lippia filifolia Mart. and Schauer ex Schauer. In Vitro Cell. Dev. Biol. Plant 42:558-561.
Crossref

 

Rotman AD, Mulgura de Romero E (1999). Verbenaceae. Flora Del Valle De Lerma. 5:1-37.

 

Sansberro PA, Mroginski LA (1995). Micropropagation of Aloysia polystachya (Verbenaceae). Agriscientia 12:83-86.

 

Tanimoto E. (2005). Regulation of root growth by plant hormones: Roles for auxin and gibberellin. Crit. Rev. Plant Sci. 24(4):249-265.
Crossref

 

Tavares E, Lopes D, Bizzo H, Lage C, Leitao S (2004). Kinetin enhanced linalool production by in vitro plantlets of Lippia alba. J. Essent. Oil Res. 16:405-408.
Crossref

 

Thorpe T, Harry I, Kumar P. (1991). Application of micropropagation to forestry. Kluwer Academic Publishers, Dordrecht, the Netherlands, pp. 311-316.
Crossref

 

Van Hellemon J (1986). Compendium de phytotherapie, Service Scientifique de l'APB, Bruxeles, Belgium, pp. 234-235.

 

Vogel H, Silva ML, Razmilic I (1999). Seasonal fluctuation of essential oil content in lemon verbena (Aloysia triphylla (L'Herit.) Britton). Acta Hortic. 500:75-79.
Crossref