Clinical Reviews and Opinions

  • Abbreviation: Clin. Rev. Opinions
  • Language: English
  • ISSN: 2141-2553
  • DOI: 10.5897/CRO
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

In vitro- and in vivo- experimental models for balanced activity of oncogenes and tumor-suppressor genes in normal and malignant cells

Iskra Ventseslavova Sainova
  • Iskra Ventseslavova Sainova
  • Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Street, 1113 Sofia, Bulgaria.
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Ilina Vavrek
  • Ilina Vavrek
  • Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Street, 1113 Sofia, Bulgaria.
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Velichka Pavlova
  • Velichka Pavlova
  • Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Street, 1113 Sofia, Bulgaria.
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Ivan Iliev
  • Ivan Iliev
  • Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Street, 1113 Sofia, Bulgaria.
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Lilija Yossifova
  • Lilija Yossifova
  • Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Street, 1113 Sofia, Bulgaria.
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Elena Gardeva
  • Elena Gardeva
  • Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Street, 1113 Sofia, Bulgaria.
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Elena Nikolova
  • Elena Nikolova
  • Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Street, 1113 Sofia, Bulgaria.
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Teodora Daneva
  • Teodora Daneva
  • Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Street, 1113 Sofia, Bulgaria.
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Roberto Nitsch
  • Roberto Nitsch
  • Institute of Molecular Biotechnology (IMBA) to Austrian Academy of Sciences, “Dr. Bohr” Street, 1030 Vienna, Austria.
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Anna Nitsch
  • Anna Nitsch
  • Institute of Molecular Biotechnology (IMBA) to Austrian Academy of Sciences, “Dr. Bohr” Street, 1030 Vienna, Austria.
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  •  Accepted: 17 June 2011
  •  Published: 30 June 2011

Abstract

Gene transfer in laboratory-cultivated mouse embryonic stem cells (mESCs) was made by appropriate recombinant DNA-constructs. Electrophorhetic profiles of genetic material from wild type (WT) on oncogene Dcn1 and “knock-down” (KD) on it inbred lines of experimental mice differed not only on it, but also on the tumor-suppressor gene HACE1 between both categories of laboratory rodents. The results obtained were compared with previous data, received from malignant rat insulinoma RIN-5F cells, transfected by recombinant gene constructs with inserted copy of secretagogin gene, by their in vitro-co-cultivation with malignant cell precursors, derived from populations of non-transfected laboratory-cultivated mESCs in the presence of doxyciclin, probably by activation of tumor-suppressor genes ofSTAT-family. These data were confirmed by the differences noticed in the degree of myeloid differentiation of derived precursor cells in their in vitro-co-cultivation with containing additional copy of secretagogin gene Rin-5F malignant rat insulinoma cells, in comparison with the results, obtained in their laboratory co-cultivation with non-treated human cervical carcinoma Hela cells, as well as with derived normal mESCs, containing additional copy of the oncogeneDcn1 as a result of their transfection with recombinant DNA-constructs. On the other hand, the derived normal cells with inserted additional copy of oncogene indicated safety, immunogenity, and they also indicated preserved normal cell characteristics.

 

Key words: Oncogenes, tumor-suppressor genes, myeloid cell precursors, recombinant gene constructs, cell transfection.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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