International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 99

Full Length Research Paper

Development of cost-effective media for the culture of Chilo partellus larvicide in Kenya

Daniel Anyika1*, Hamadi Boga2 and Romano Mwirichia1
1Institute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology (JKUAT),P.O. Box 62000, Nairobi, 00200, Kenya. 2Department of Botany, Faculty of Science, Jomo Kenyatta University of Agriculture and Technology (JKUAT), P.O. Box 62000, Nairobi, 00200, Kenya.
Email: [email protected]

  •  Accepted: 26 February 2013
  •  Published: 31 March 2013

Abstract

Stem borers (Chilo partellus) are important field insect pests of maize (Zea mays L.) and sorghum (Sorghum bicolor L.) in Africa. They account for more than 30% yield losses depending on the composition of the pest community. C. partellus larvicide like Bacillus thuringiensis have been widely and effectively used in C. partelluscontrol programs, but the industrial production of theses bacilli is expensive. Here we have attempted to develop three cost-effective media, based on legumes, potato, and whey. Growth and production of the insecticidal proteins from these bacteria were satisfactory; protein concentration yields of 27.60 mg/ml, spore counts of 5.60 × 108 CFU/ ml and first-instar Chilo partellus larvicidal activity (LC50) of 78 µg/l were obtained with a 72 h culture of this bacterium. Therefore, this investigation suggests that legume, potato and whey-based culture media are more economical and effective for the industrial production of B. thuringiensis insecticidal crystal proteins.

Key words: Bacillus thuringiensis, larvicidal, Chilo partellus, insecticidal crystal proteins, LC50, optical density,sulphate polyacrylamide gel electrophoresis (SDS-PAGE), spore counts.