Abstract

Several studies have been reported on the bla_TEM, bla_CTX-M and bla_SHV genes in Extended-spectrum β-lactamase (ESBL) producing Enterobacteria, however very few studies reported in the literature are related to bla_BES in ESBL producing Enterobacteria. This study concerns the molecular epidemiology of the bla_BES gene in Enterobacteria identified from in-patients and out-patients at Saint Camille hospital of Ouagadougou (Burkina Faso). The study was first involved microbiological identification of Enterobacteria that are implicated in antibiotic resistance using API 20 E system; the antibiotics susceptibility test was secondly performed by the diffusion method and the molecular characterization was finally made by PCR to detect the bla_BES gene. Data were entered and analyzed using Excel 2013 and EPI Info version 6.0 software. A p-value < 0.05 was considered as significant. A total of 60 isolates of ESBL-producing Enterobacteria were found: 21 (35%) Escherichia coli; 18 (30%) Klebsiella pneumoniae; 6 (10%) Enterobacter cloacae; 4 (7%) Proteus mirabilis; 4 (7%) Serratia marcescens; 3 (5%) Citrobacter freundii; 1 (1.6%) Enterobacter aerogenes; 1 (1.6%) Citrobacter brakii; 1 (1.6%) Citrobacter youngae and 1 (1.6%) Salmonella arizonae. Molecular characterization revealed the presence of the bla_BES gene in 38 (63.3%) of bacterial isolates carried by patients. The presence of bla_BES gene in ESBL producing Enterobacteria at Saint Camille Hospital in Ouagadougou was therefore established in this study for the first time in Burkina faso.

Key words: Enterobacteria, Extended-spectrum β-lactamase (ESBL), bla_BES, gene, hospital, resistance, Ouagadougou.