International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 98

Full Length Research Paper

First detection of the BES-type Extended-Spectrum β-Lactamase produced by Enterobacteria at Saint Camille Hospital of Ouagadougou (Burkina Faso)

Sougué Serge
  • Sougué Serge
  • Department of Biochemistry and Microbiology, Faculty of life and earth Science, University Joseph KI-ZERBO of Ouagadougou, National Public Health Laboratory of Ouagadougou, Burkina Faso.
  • Google Scholar
Mètuor-Dabiré Amana
  • Mètuor-Dabiré Amana
  • Laboratory of Biology and Molecular Genetics (LABIOGENE), Pietra Annigonni Biomolecular Research Center (CERBA), University of Dédougou, Burkina Faso.
  • Google Scholar
Zohoncon Théodora Mahoukèdè
  • Zohoncon Théodora Mahoukèdè
  • Saint Camille Hospital of Ouagadougou, Saint Thomas D’Aquin University of Ouagadougou, Burkina Faso.
  • Google Scholar
Tiemtoré Rahimatou Yasmine Wend-kuni
  • Tiemtoré Rahimatou Yasmine Wend-kuni
  • Department of Biochemistry and Microbiology, Faculty of life and earth Science, University Joseph KI-ZERBO of Ouagadougou, National Public Health Laboratory of Ouagadougou, Burkina Faso.
  • Google Scholar
Bangré Yasmine Aminata
  • Bangré Yasmine Aminata
  • Department of Biochemistry and Microbiology, Faculty of life and earth Science, University Joseph KI-ZERBO of Ouagadougou, National Public Health Laboratory of Ouagadougou, Burkina Faso.
  • Google Scholar
Zongo Jacob Koudbi
  • Zongo Jacob Koudbi
  • Laboratory of Biology and Molecular Genetics (LABIOGENE), Pietra Annigonni Biomolecular Research Center (CERBA), University of Dédougou, Burkina Faso.
  • Google Scholar
Simporé Jacques
  • Simporé Jacques
  • Department of Biochemistry and Microbiology, Faculty of life and earth Science, University Joseph KI-ZERBO of Ouagadougou, National Public Health Laboratory of Ouagadougou, Burkina Faso.
  • Google Scholar


  •  Received: 21 May 2019
  •  Accepted: 02 August 2019
  •  Published: 31 August 2019

Abstract

Several studies have been reported on the blaTEM, blaCTX-M and blaSHV genes in Extended-spectrum β-lactamase (ESBL) producing Enterobacteria, however very few studies reported in the literature are related to blaBES in ESBL producing Enterobacteria. This study concerns the molecular epidemiology of the blaBES gene in Enterobacteria identified from in-patients and out-patients at Saint Camille hospital of Ouagadougou (Burkina Faso). The study was first involved microbiological identification of Enterobacteria that are implicated in antibiotic resistance using API 20 E system; the antibiotics susceptibility test was secondly performed by the diffusion method and the molecular characterization was finally made by PCR to detect the blaBES gene. Data were entered and analyzed using Excel 2013 and EPI Info version 6.0 software. A p-value < 0.05 was considered as significant. A total of 60 isolates of ESBL-producing Enterobacteria were found: 21 (35%) Escherichia coli; 18 (30%) Klebsiella pneumoniae; 6 (10%) Enterobacter cloacae; 4 (7%) Proteus mirabilis; 4 (7%) Serratia marcescens; 3 (5%) Citrobacter freundii; 1 (1.6%) Enterobacter aerogenes; 1 (1.6%) Citrobacter brakii; 1 (1.6%) Citrobacter youngae and 1 (1.6%) Salmonella arizonae. Molecular characterization revealed the presence of the blaBES gene in 38 (63.3%) of bacterial isolates carried by patients. The presence of blaBES gene in ESBL producing Enterobacteria at Saint Camille Hospital in Ouagadougou was therefore established in this study for the first time in Burkina faso.

 

Key words: Enterobacteria, Extended-spectrum β-lactamase (ESBL), blaBES, gene, hospital, resistance, Ouagadougou.