International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 103

Full Length Research Paper

A quick bud breaking response of a surface model for rapid clonal propagation in Centella asiatica (L.)

AK Bhandari1*, M Baunthiyal2, VK Bisht1, Narayan Singh1 and JS Negi1
1Herbal Research and Development Institute (HRDI) - Mandal, Gopeshwar, Chamoli, Uttarakhand, India. 2Department of Biotechnology, G. B. Pant Engineering College- Ghurdauri, Pauri-Garhwal, Uttarakhand, India.
Email: [email protected]

  •  Accepted: 09 September 2013
  •  Published: 31 October 2013


Present investigation was planned to evaluate time period of bud breaking in Centella asiatica with different concentration of plant growth regulators, a medicinal herb distributed throughout the worldwide. For the study, concentrations were designed for response surface model describing bud breaking growth in optimum conditions. A combination of BAP (2 mg/L) + gibberellic acid (GA3, 0.5 mg/L) was achieved at a best initial bud breaking at 8thhour. Longest time period taken for bud breaking was shown in combination of BAP (0.5 mg/L) + naphthalene acetic acid (NAA, 0.5 g / L) and BAP (0.1 mg/L) + adenine sulphate (0.5 mg/L) which was recorded at 84th hour. Half strength MS media was supplemented with IBA alone (2 mg/L) and in combination with IAA (0.5 mg/L) to attain an early in vitro rooting. Their interactions observed were statistically significant (P < 0.05).


Key words: Centella asiatica, bud, plant growth regulator, medicinal plant.


MS, Murashige; BAP, 6-benzylaminopurine; NAA, naphthaleneacetic acid; GA3, gibberellic acid;IBA, indole-3-butyric acid, Kn, kinetin; IAA, indole-3 acetic acid; PGR, plant growth regulator; ANOVA, analysis of variance.