International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 105

Full Length Research Paper

Beta glucuronidase activity in early stages of rice seedlings and callus: A comparison with Escherichia coli beta glucuronidase expressed in the transgenic rice

Poosakkannu Anbu1,2* and Loganathan Arul1
1Department of Plant Molecular Biology and Biotechnology, Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore-641 003, India. 2Department of Biological and Environmental Sciences, University of Jyväskylä, P.O. Box, 35, FIN-40014, Finland.
Email: [email protected]

  •  Accepted: 30 July 2013
  •  Published: 30 August 2013

Abstract

We have chosen rice as a model crop to ascertain endogenous β-glucuronidase (family 79) activity and to differentiate the same from Escherichia coli based β-glucuronidase (family-2). The investigation dwells on characterizing endogenous β-glucuronidase (GUS) activity during the early stages of seed germination and from rice callus. Also, similar studies were made from homozygous transgenic rice line expressing E. coli GUS under the control of glyoxalase I promoter. Endogenous GUS activity was detected in plumules, shoots and calli of rice, nevertheless, showing differential response to pH. Further, endogenous GUS in rice was specifically inhibited by saccharic acid 1,4-lactone (SL) but, the E. coli β-glucuronidase remain unaffected, indicating distinct biochemical properties of family-2 and family-79 β-glucuronidase.

Key words: Family 2, family 79, X-gluc, saccharic acid, 1,4-lactone.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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