International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 99

Full Length Research Paper

In vitro propagation of two elite cooking banana cultivars- FHIA 17 and INJAGI

Jane Kahia*
  • Jane Kahia*
  • World Agroforestry Centre (ICRAF), Cote d’ Ivoire Country Program Cocody Mermoz,Abidjan, 08 BP 2823, Côte d’Ivoire.
  • Google Scholar
Felicien Ndaruhutse
  • Felicien Ndaruhutse
  • Department of Crop Production and Horticulture, Faculty of Agriculture, National University of Rwanda, P.O. Box117, Huye, Rwanda.
  • Google Scholar
Bancy Waweru3
  • Bancy Waweru3
  • Rwanda Agriculture Board (RAB), P.O. Box 5016, Kigali, Rwanda.
  • Google Scholar
Ntirugulirwa Bonaventure
  • Ntirugulirwa Bonaventure
  • Rwanda Agriculture Board (RAB), P.O. Box 5016, Kigali, Rwanda.
  • Google Scholar
Amini Mutaganda
  • Amini Mutaganda
  • Rwanda Agriculture Board (RAB), P.O. Box 5016, Kigali, Rwanda.
  • Google Scholar
Peter Yao Sallah
  • Peter Yao Sallah
  • Department of Crop Production and Horticulture, Faculty of Agriculture, National University of Rwanda, P.O. Box117, Huye, Rwanda.
  • Google Scholar
Njenga Peter Kariuki
  • Njenga Peter Kariuki
  • Jomo Kenyatta University of Agriculture and Technology, P.O Box 62000, Nairobi, Kenya.
  • Google Scholar
Theodore Asiimwe
  • Theodore Asiimwe
  • Rwanda Agriculture Board (RAB), P.O. Box 5016, Kigali, Rwanda.
  • Google Scholar


  •  Received: 07 May 2015
  •  Accepted: 19 October 2015
  •  Published: 30 November 2015

Abstract

Explants of two elite cooking banana cultivars, FHIA17 and INJAGI were collected from healthy source of stock plants growing in the field. Sterilization was evaluated using different concentrations (20, 30, 40, 50 and 60%) of a commercial bleach (JIK) for 25 min. Effects of cytokinins benzyl amino purine (BAP), 2-isopentenyl adenine (2iP) and kinetin each evaluated at 5, 10, 20, 24 and 40 µM and thidiazuron (TDZ) at 0.1, 0.5, 1 and 1.5 µM, on microshoot induction were investigated. Effects of auxins viz. napthaleneacetic acid (NAA), indole-3-acetic acid (IAA), 2,4-dichlorophenoxyacetic acid (2,4-D) and indole-3-butyric acid (IBA each evaluated at 5, 10, 20 and 40 µM) on rooting were tested as well. The highest (91%) numbers of clean explants were obtained when the explants were subjected to sterilization in 40% Jik for 25 min. The FHIA 17 explants cultured on MS media supplemented with 40 µM kinetin produced the highest (3.00±0.35) mean number of microshoots. On the other hand, INJAGI explants cultured on MS media supplemented with 0.1 µM TDZ yielded the highest mean number (1.84±0.24) of microshoots and the highest mean length (0.99±0.13). Rooting was achieved in all media supplemented with the auxins evaluated except on 2,4-D. These results have an important significance on the application of tissue culture in propagation of cooking bananas in Rwanda, which is highly desired to support the government policy of replacing old unproductive bananas with elite high yielding varieties. 
 
Key words: FHIA17, INJAGI, banana, microshoot, cultivar.