International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 100

Full Length Research Paper

Screening of different insect pests of mulberry and other agricultural crops for microsporidian infection

Ifat Bashir1*, S. D. Sharma2 and Shabir A. Bhat3
1Additional Directorate of Sericulture Development Department, Tulsi Bagh, Srinagar- 190 001 (J&K), India. 2Central Sericultural Research and Training Institute Srirampura Mysore- 570008, India. 3Division of Sericulture, Mirgund, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Post Box: 674,GPO Srinagar- 190001, India.    
Email: [email protected]

  •  Accepted: 05 August 2011
  •  Published: 31 August 2011

Abstract

In the present study, different insect pests of mulberry and other agricultural crops were collected from mulberry gardens and agricultural crop fields in and around Mysore, Karnataka, India. The collected insects were screened for microsporidian infection and microsporidian spores were isolated from infected specimens, purified and tentatively designated as NIK- 1Pr, NIK- 1Cc, NIK- 1Cpy, NIK- 1So and NIK- 1Dp. The serological affinity test carried out through monoclonal and polyclonal antibodies, showed negative reaction of these microsporidia which indicated that these are niether Nosema bombycis nor Lbms.

Key words:  Agricultural crops, mulberry, pests, microsporidia, monoclonal and polyclonal antibodies.