International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 100

Table of Content: June 2012; 3(3)

June 2012

Enhancing thermostability of the biocatalysts beyond their natural function via protein engineering

Majority of the naturally occurring enzymes lacks essential features required during the harsh conditions of the industrial processes, because of their less stability. Protein engineering tool offers excellent opportunity to improve the biochemical properties of these biocatalysts. These techniques further help in understanding the structure and function of the proteins. Most common methods employed in protein...

Author(s): Shelly Goomber, Pushpender K. Sharma, Monika Sharma, Ranvir Singh and Jagdeep Kaur

June 2012

Extraction, purification and characterization of L-asparaginase from Penicillium sp. by submerged fermentation

A fungal strain identified as Penicillium sp. was evaluated for its L–asparaginase enzyme production. The L-asparaginase enzyme was purified to homogeneity from Penicillium sp. that was grown on submerged fermentation. Different purification steps including salt precipitation, followed by separation on sephadex G-100-120 gel filtration and DEAE were applied to obtain pure enzyme preparation....

Author(s): Krishna Raju Patro and Nibha Gupta