International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 97

Full Length Research Paper

Extraction, purification and characterization of L-asparaginase from Penicillium sp. by submerged fermentation

Krishna Raju Patro and Nibha Gupta*
Regional Plant Resource Centre, Bhubaneswar, 751 015 Odisha, India.
Email: [email protected]

  •  Accepted: 02 May 2012
  •  Published: 30 June 2012

Abstract

A fungal strain identified as Penicillium sp. was evaluated for its L–asparaginase enzyme production. The L-asparaginase enzyme was purified to homogeneity from Penicillium sp. that was grown on submerged fermentation. Different purification steps including salt precipitation, followed by separation on sephadex G-100-120 gel filtration and DEAE were applied to obtain pure enzyme preparation. The purified enzyme showed 13.97 IU/mg specific activity and 36.204% yield. The polyacrylmide gel electrophoresis of the pure enzyme exhibited one protein of 66 kDa. The enzyme showed maximum activity at 7.0 pH and 37°C and Km value 4.00 × 10-3 M.

Key words: L – Asparaginase, fungi, Penicillium.

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