International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 131

Full Length Research Paper

Application of multiple displacement amplification in detection of five events of genetically modified organisms

Xi Zhou1, Hongwei Gao2, Liqing Zhao2, Wenda Liu1, Min Sun2 and Shubai Wang1*
1Qingdao Agricultural University, No. 700, Changcheng Road, Chengyang, Qingdao, P. R. China. 2Shandong Entry-Exit Inspection and Quarantine Bureau of People’s Republic of China. No.70, Qutangxia Road, Qingdao City, 266002, Shandong, P. R. of China.
Email: [email protected]

  •  Accepted: 24 October 2013
  •  Published: 31 December 2013

Abstract

In this study, multiple displacement amplification (MDA) was used for transgene detection, and proved to effectively improve the sensitivity of most fluorescence polymerase chain (PCR). Five events of genetically modified plants were chosen for detection in different plant species by MDA and real-time PCRs. Individual primer/probe systems based on each transgene target were used. The extracted DNA of each sample was diluted and used as a template for MDA. All extracted DNA and diluted mdaDNA were detected by real-time PCR. The results were evaluated with respect to DNA quantity, DNA purity and difference of Ct values of the isogenetic DNA extract before and after MDA (D-values). The MDA amplifying effects were largely different among genes and plants. Organelle DNA has more copies than genomic DNA by MDA.

Key words: Multiple displacement amplification, genetically modified organisms, detection, events.