International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 131

Full Length Research Paper

Rapid detection of Salmonella species in newborne calves by polymerase chain reaction

I. M. Moussa1*, M. H. Ashgan2, M. S. Mohamed3, K. H. F. Mohamed4 and A. A. Al-Doss1
  1Center of Excellence in Biotechnology, King Saud University, P. O. Box 2460 Riyadh, King Saudi Arabia. 2College of Applied Studies and Community Service, King Saud University, P. O. Box 2460 Riyadh, King Saudi Arabia. 3Department of Microbiology, Riyadh Colleges for Dentistry and Pharmacy, Riyadh, King Saudi Arabia. 4Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Email: [email protected] , [email protected]

  •  Accepted: 05 March 2010
  •  Published: 30 April 2010

Abstract

 

Fecal samples collected from 85 diarrheic calves and 65 apparently healthy contact calves were examined for the presence of Salmonella species using bacteriological examination and fimA gene amplification assay (PCR). Salmonella were isolated from 43.53% of diarrheic calves and from 27.69% of apparently healthy contact calves.Salmonella typhimurium were isolated from diarrheic and contact calves in percentages of 17.65 and 15.38% respectively; whereas Salmonella entertidis were isolated in percentages of 11.76 and 7.69%, respectively. Salmonella dublin were isolated from the diarrheic calves in a percentage of (8.24%) and contact calves in a percentage of (4.62%), but Salmonella anatum were isolated from diarrheic calves only in a percentage of (5.88%), The sensitivity and specificity of PCR were 100 and 95.8%, respectively during examination of fecal samples obtained from diarrheic calves, while during the examination of those obtained from contact calves the percentages were 100 and 89.4%, respectively.

 

Key words: Salmonella, fimA gene, diarrheic calves, Salmonella typhimurium, Salmonella entertidis.