International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 131

Full Length Research Paper

Mapping Rf3 locus in rice by SSR and CAPS markers

M. Alavi1*, A. Ahmadikhah1, B. Kamkar2 and M. Kalateh3
  1Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. 2Department of Agronomy, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. 3Agricultural Selection Station, Gorgan, Iran.
Email: [email protected]

  •  Accepted: 11 September 2009
  •  Published: 31 October 2009

Abstract

 

Cytoplasmic male sterility (CMS) is a common phenomenon that has been extensively used for production of hybrid seeds in various crops. Rf genes are needed for restoring fertility to CMS lines. Searching for and molecular tagging of restorer genes is of high importance where phonotyping is very time consuming and requires the determination of spikelet sterility in testcross progeny. In this study we attempted to map a fertility restorer gene using SSR and CAPS markers in rice line IR36 in a F2 population developed from the cross Neda-A×IR36. The genetic linkage analysis indicated that thee SSR markers (RM1, RM3233, RM3873) and one CAPS marker (RG140/EcoRI) on the short arm of chromosome 1 were linked to Rf3Rf3 flanked by tow SSR markers RM1 and RM3873 at distances of 5.6 and 14 cM, respectively. The use of identified markers give promise for their application in molecular Marker Assisted Selection (MAS).

 

Key words: CMS, fertility restoration, SSR marker, Rf3 gene.