International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 131

Amplification and sequencing of Rosaceae expressed sequence tags (ESTs) as a resource for functional genomics databases

Haddad El Rabey1* and Francesca Barale2
  1Genetic Engineering and Biotechnology Institute, Minufiya University, P. O. Box 79 Sadat City, Egypt. 2Faculty of Agriculture, Milan University, Milan, Italy.
Email: [email protected]

  •  Accepted: 16 February 2010
  •  Published: 30 April 2010

Abstract

 

A total of 30 successful ESTs (expressed sequenced tags) were amplified and sequenced to be intended as a resource for Rosaceae functional genomics data base. 23 EST were isolated from the amplification of Earlygold peach (Prunus persica) cultivar DNA, 5 ESTs were isolated from the amplification of Texas almond cultivar and two ESTs were isolated from the amplification of F1 DNA of their hybrid. All the sequences were tested for similarity using blast in the nbci database. Because these sequence data are new, only 13 sequences found similarity (10 belong to Earlygold peach cv., two belong to Texas almond cultivar and one belongs to their F1 hybrid), whereas the other 17 (13 belong to Earlygold peach cv., three belong to Texas almond cultivar and one belongs to their F1 hybrid) found no significant similarity. The resulting database can be used as a resource of data and links related to peach and almond EST sequences. 

 

Key words: Peach, almond, expressed sequence tags, functional genomics.