International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 131

Full Length Research Paper

Genetic typing and in silico assignment of smoked and fresh bushmeat sold on markets and restaurants in west-central Côte d'Ivoire

Félix Kouadio Yéboué
  • Félix Kouadio Yéboué
  • Université Jean Lorougnon Guédé, UFR Environnement, Laboratoire de Biodiversité et Gestion durable des Ecosystèmes Tropicaux, Unité de Recherche en Génétique et Epidémiologie Moléculaire (URGEM), BP 150 Daloa, Côte d’Ivoire.
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Mathurin Koffi
  • Mathurin Koffi
  • Université Jean Lorougnon Guédé, UFR Environnement, Laboratoire de Biodiversité et Gestion durable des Ecosystèmes Tropicaux, Unité de Recherche en Génétique et Epidémiologie Moléculaire (URGEM), BP 150 Daloa, Côte d’Ivoire.
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Innocent Allepo Abe
  • Innocent Allepo Abe
  • Université Jean Lorougnon Guédé, UFR Environnement, Laboratoire de Biodiversité et Gestion durable des Ecosystèmes Tropicaux, Unité de Recherche en Génétique et Epidémiologie Moléculaire (URGEM), BP 150 Daloa, Côte d’Ivoire.
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Martial Kassi N’Djetchi
  • Martial Kassi N’Djetchi
  • Université Jean Lorougnon Guédé, UFR Environnement, Laboratoire de Biodiversité et Gestion durable des Ecosystèmes Tropicaux, Unité de Recherche en Génétique et Epidémiologie Moléculaire (URGEM), BP 150 Daloa, Côte d’Ivoire.
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Thomas Konan Konan
  • Thomas Konan Konan
  • Université Jean Lorougnon Guédé, UFR Environnement, Laboratoire de Biodiversité et Gestion durable des Ecosystèmes Tropicaux, Unité de Recherche en Génétique et Epidémiologie Moléculaire (URGEM), BP 150 Daloa, Côte d’Ivoire.
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Béné Jean-Claude Koffi
  • Béné Jean-Claude Koffi
  • Université Jean Lorougnon Guédé, UFR Environnement, Laboratoire de Biodiversité et Gestion durable des Ecosystèmes Tropicaux, Unité de Recherche en Primatologie, BP 150 Daloa, Côte d’Ivoire.
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  •  Received: 17 November 2020
  •  Accepted: 13 January 2021
  •  Published: 31 January 2021

Abstract

Poachers and bushmeat traders most of the time modify the wildlife materials before commercialization. Therefore, the morphological identification of species is no longer possible. However, accurate identification of the sale wildlife materials is essential for species identification, quantification and better conservation management. The aim of this study is to take advantages of specific gene sequences and in silico analysis to identify smoked and decapitated wildlife materials encountered on bushmeat markets and traditional restaurants in Center-Western Côte d’Ivoire. DNA from 352 bushmeat samples comprising 123 decapitated and smoked specimens was extracted, amplified by polymerase chain reaction (PCR) using the Cytb and COI primers and analyzed in silico. Up to 98.37% of the DNA from smoked bushmeat was successfully amplified. Assignment of Cytb and COI query sequences with reference sequences in the NCBI Genbank allowed to correct three misidentifications by traders. Thus, specimens identified as Heliosciurus sp, Phacochoerus aethiopicus africanus and Dendrohyrax dorsalis sylvestris by traders were assigned to Funisciurus sp, Tragelaphus scriptus, Crossarchus obscurus with 84.94, 100, 98.76% similarity respectively. Morphologically unidentifiable sample coded IP3CS1O was assigned to species Eidolon helvum with 99.53% similarity.  The study shows the interest of DNA-based identification of bushmeat to circumvent misidentification for better management of wildlife biodiversity.

 

Key words: smoked bushmeat, misidentification, genetic typing, in silico analysis, identification, biodiversity, wildlife conservation.