Abstract

Coexistence between the TOHO-type and Brazilian extended-spectrum (BES)-type of extended-spectrum β-lactamase (ESBL)-produced by bacteria caused public health issue. Several studies have been reported on the coexistence between blaTEM, blaCTX-M and blaSHV genes in ESBL in broad spectrum enterobacteria. The present study involved the prevalence of coexistence of blaTOHO and blaBES genes in enterobacteria identified in hospitalized and out-patients at Saint Camille Hospital in Ouagadougou (Burkina Faso). Firstly, the study was led by microbiological identification of enterobacteria, secondly antibiogram was performed by diffusion method and finally the molecular characterization was done by conventional polymerase chain reaction (PCR) to search for antibiotic resistance genes blaTOHO and blaBES. The ultraviolet (UV) lamp (Gene Flash) for the photography of gels allowed the visualization of specific bands of TOHO and BES genes. Among 250 strains of Gram negative bacilli collected, 60 strains (24.1%) showed resistance profile to antibiotics used. Molecular characterization showed the coexistence between blaTOHO and blaBES genes in 53.3% in bacteria strains carried by the patients. The highest prevalence was observed in Escherichia coli (34.4%) and Klebsiella pneumoniae (21.9%) strains. For the first time in Ouagadougou, Burkina Faso, this study therefore established the coexistence between blaTOHO and blaBES genes in ESBL produced enterobacteria at Saint Camille Hospital.

Key words:  Antibiotic, resistance, extended-spectrum β-lactamase (ESBL), genes, TOHO, Brazilian extended-spectrum (BES).