International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 131

Full Length Research Paper

Lack of association between - 308 tumor necrosis factor polymorphism and susceptibility to cerebral malaria among Central Sudanese children

Adil Mergani1, Ammar H. Khamis2, Ali Babikir Haboor3, Elfatih Hashim4, Mohammed Gumma5, Bella Awadelseed4, M. M. Magzoub7, Ahmed Al Tahir6 and Nasreldin M. A. Elwali7
  1Faculty of Applied Medical Sciences, Taif University, Kingdom of Saudi Arabia. 2Sudan University for Science and Technology, Khartoum, Sudan. 3 Faculty of Medicine, University of Gezira, Wad Medani, Sudan. 4Faculty of Medicine, University of Sinnar, Sinnar, Sudan. 5Singa Hospital, Ministry of Health, Sudan. 6Faculty of Medicine, King Fahad Medical City, Kingdom of Saudi Arabia. 7Institute of Nuclear Medicine, Molecular Biology and Oncology, University of Gezira, Wad Medani, Sudan.
Email: [email protected]

  •  Accepted: 13 April 2010
  •  Published: 30 May 2010

Abstract

 

Up to 500 millions are affected by malaria parasite each year and only 1% of them develop severe clinical forms of the disease. No full satisfactory explanation for the fact that only small proportion of malaria infected individuals develops severe clinical phenotypes while others don’t. The aims of the present study is to assess the role of TNF-α - 308 G>A in predisposing to cerebral malaria in children in Central Sudan. 109 children admitted with cerebral malaria (CM) were enrolled in this study (Mean age 6.1± 3.3 years old). The onset of cerebral malaria in the study subjects started in the first two years of life with no gender effect and the highest incidence of the disease was at the age group (4 - 6 years old). The allele frequencies in control group (n= 109) were 0.91 for TNFα-308G (TNF1) and 0.09 for TNFα-308A (TNF2) and the allele frequencies in 93 CM subjects were 0.95 for TNFα-308G (TNF1) and 0.05 for TNFα-308A (TNF2). The distribution of TNFα -308 genotypes in normal group was consistent with the Hardy-Weinberg equilibrium. No TNF2 homozygote was observed among CM subjects. However, the distribution of TNFα-308 genotypes and alleles did not differ significantly between CM patients and controls (P = 0.271). These data suggest that TNF2 is not associated with predisposition to CM in Central Sudanese children. Further studies for confirmation of this finding in other regions of Sudan are required.

 

Key words: Cerebral malaria, genetic susceptibility, cytokine, TNF.