Thirteen amphiploids were synthesized by chromosome doubling of an F1 plant of a cross between Triticum aestivum Mianyang11 × S. cereale Kustro, which shows resistance to Bird cherry-oat aphid (Rhopalosiphum padi). The amphiploids were confirmed by GISH analysis and tested for their reaction to R. padi. Eleven amphiploids exhibited resistance to R. padiand two of them exhibited high level of susceptibility. The PCR analysis using rye-specific markers provided evidence for the elimination of W-box elements from the susceptible amphiploids. The possible mechanism of loss resistance in some amphiploids was discussed. The resistant amphiploids can be exploited in wheat breeding programs to transfer R. padi resistance to susceptible wheat varieties.
Key words: Rhopalosiphum padi, amphiploid, wheat, rye.
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