International Journal of
Livestock Production

  • Abbreviation: Int. J. Livest. Prod.
  • Language: English
  • ISSN: 2141-2448
  • DOI: 10.5897/IJLP
  • Start Year: 2009
  • Published Articles: 263

Full Length Research Paper

Isolation of some respiratory viruses from camels

Intisar, K. S.
  • Intisar, K. S.
  • Central Veterinary Research Laboratory, P. O. Box 8067, Al Amarat, Khartoum, Sudan.
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Ali, Y. H.
  • Ali, Y. H.
  • Central Veterinary Research Laboratory, P. O. Box 8067, Al Amarat, Khartoum, Sudan.
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Taha, K. M.
  • Taha, K. M.
  • Atbara Veterinary research Laboratory, River Nile State, Sudan.
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Musa, M. Z.
  • Musa, M. Z.
  • Camel Research Center, Tambool, Gezira State, Sudan.
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Khalafalla, A. I.
  • Khalafalla, A. I.
  • Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum, Khartoum, Sudan.
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  •  Accepted: 18 October 2013
  •  Published: 31 January 2014

Abstract

The presence of three of the main respiratory viruses found in camels, that is, peste des petits ruminants virus (PPRV), bovine para-influenza virus 3 (PIV3) and bovine herpesvirus-1 (BHV-1), was investigated and isolation of these viruses in different cell cultures was carried out. A total of 100 pneumonic camel lungs collected from slaughterhouses were screened for the presence of PPR, PIV3 and BHV-1 viral antigens using ELISA. Forty five were found to be positive for PPRV, 15 for PIV3 and 4 for BHV-1 virus antigen. Of these samples, 15 PPR, 12 PIV3 and 3 BHV-1 positives were inoculated in MDBK cell line, primary and secondary lamb and bovine kidney cells. Each of the three viruses were successfully isolated in the different cultures used; CPE was seen at day 14 for PPR, day 3 for PIV3 and day 2 for BHV-1 after infection; CPE was observed, characterized by cell rounding, elongation with some syncytia formation for both PPR and PIV3, and BHV-1  samples showed cell rounding, edematous cells and cell sheet detachment. This is the first report of the isolation of PPR virus from camels.

Key words: Camels, cell cultures, respiratory viruses, Sudan.