International Journal of
Nutrition and Metabolism

  • Abbreviation: Int. J. Nutr. Metab.
  • Language: English
  • ISSN: 2141-2332
  • DOI: 10.5897/IJNAM
  • Start Year: 2009
  • Published Articles: 125

Full Length Research Paper

Isolation and proximate determination of protein using defatted sesame seed oil cake

Ranganayaki, S.
  • Ranganayaki, S.
  • Department of Biotechnology, Dr. Mahalingam Centre for Research and Development, N.G.M. College, Pollachi 642001, India.
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Vidhya, R.
  • Vidhya, R.
  • SRF, Department of Food Product Development, India.
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Jaganmohan, R.
  • Jaganmohan, R.
  • Indian Institute of Crop processing Technology, Pudukottai Road, Thanjavur – 613005, Tamilnadu, India.
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  •  Accepted: 12 March 2012
  •  Published: 30 November 2012


Sesame seed was used to extract oil and the cake, which has high amount of protein, was wasted after the extraction. Hence, the present study was done to isolate protein from defatted sesame seed oil cake and the nutrition content was determined. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) was used to isolate the protein; among the three BSS (British Standard Sieve) of the sesame seed flour cake, 52 BSS shows high protein content of 45.9% while 72 and 32 BSS contains 30.3 and 33.4% of protein, respectively. Low fat content of 4.4% was observed in 52 BSS which showed higher fiber content of 3.8%. The moisture content of 52 BSS was found to be 6.8% and others of 7.0 and 7.2%, respectively.


Key words: Protein isolation, sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS PAGE), British standard sieve.