International Journal of
Plant Physiology and Biochemistry

  • Abbreviation: Int. J. Plant Physiol. Biochem.
  • Language: English
  • ISSN: 2141-2162
  • DOI: 10.5897/IJPPB
  • Start Year: 2009
  • Published Articles: 110

Full Length Research Paper

The costunolide biosynthesis enzymes of Artemisia glabella Kar. et Kir.: Determination of the nucleotide sequences of the mRNA

Adekenova Gulimzhan S.
  • Adekenova Gulimzhan S.
  • JSC “International Research and Production Holding Phytochemistry, 100009, Republic of Kazakhstan.
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Shaikenova Zh. S.
  • Shaikenova Zh. S.
  • JSC “International Research and Production Holding Phytochemistry, 100009, Republic of Kazakhstan.
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Chervyakova ?. V.
  • Chervyakova ?. V.
  • Research Institute for Biological Safety Problems, Ministry of Education and Science, Republic of Kazakhstan.
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Zakarya K. D.
  • Zakarya K. D.
  • Research Institute for Biological Safety Problems, Ministry of Education and Science, Republic of Kazakhstan.
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?dekenov S. ?.
  • ?dekenov S. ?.
  • JSC “International Research and Production Holding Phytochemistry, 100009, Republic of Kazakhstan.
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  •  Received: 08 December 2020
  •  Accepted: 28 April 2021
  •  Published: 30 April 2021

Abstract

Artemisia glabella Kar. et Kir. is a source of sesquiterpene lactone arglabin, which has antitumor, radiosensitizing and immunomodulatory activity. Studying the biosynthesis of arglabin and its derivatives will allow us to develop the biotechnological basis for its production, thereby increasing its availability. The precursor to most sesquiterpene lactones is costunolide. The purpose of these studies was to detect and determine the nucleotide sequences of mRNA enzymes germacrene A synthase (GAS, EC 4.2.3.23), germacrene A oxidase (GAO, EC 1.14.14.95) and costunolide synthase (COS, EC 1.14.13.120), involved in the biosynthesis of costunolide in A. glabella. As a result of studies, mRNA was isolated from various forms (intact plant, regenerant plant, callus) of A. glabella. Using specific primers, mRNA fragments of genes encoding sesquiterpene lactones biosynthesis enzymes were amplified and their nucleotide sequences were determined. A comparative analysis of the obtained sequences showed their high (>90%) identity with the genes of GAS, GAO and COS of other representatives of the family Asteraceae. It was revealed that enzymes are expressed both in an intact plant and in calluses and regenerant plants obtained in vitro.

 

Key words: Artemisia glabella Kar. et Kir., mRNA, cDNA, sequencing, germacrene A synthase, germacrene A oxidase, costunolide synthase.