July 2011

Comparative study of the major components of the indigo dye obtained from Strobilanthes flaccidifolius Nees. and Indigofera tinctoria Linn.

Investigation on the chemical constituents including the chemical structure of major components in the water extracts from Strobilanthes flaccidifolius Nees. and Indigofera tinctoria Linn. was done. It was found that the indigo from fresh leaves of S. flaccidifolius Nees.fermented for three days gave the highest amount of indigo. The thin layer chromatography of the crude extract revealed...

Author(s): Warjeet S. Laitonjam and Sujata D. Wangkheirakpam

July 2011

Purification and some properties of b-amylase from the nodes of sugar cane, Saccharium offinacium

b-amylase (EC 3.2.1.2, a-1,4–D-glucan maltohydrolase) was isolated and purified from the nodes of sugar cane by using ammonium sulphate precipitation, acid-treatment, gel filtration on Sephadex G-75 and ion exchange chromatography on DEAE-Cellulose.  Purity was ascertained by the presence of a single band of protein on polyacrylamide gel electrophoresis under non-denaturing conditions. The specific...

Author(s): Oyefuga O. H., Adeyanju M. M., Adebawo. O. O. and Agboola F. K.