International Journal of
Plant Physiology and Biochemistry

  • Abbreviation: Int. J. Plant Physiol. Biochem.
  • Language: English
  • ISSN: 2141-2162
  • DOI: 10.5897/IJPPB
  • Start Year: 2009
  • Published Articles: 113

Full Length Research Paper

Comparative study of the major components of the indigo dye obtained from Strobilanthes flaccidifolius Nees. and Indigofera tinctoria Linn.

Warjeet S. Laitonjam* and Sujata D. Wangkheirakpam
Department of Chemistry, Manipur University, Canchipur – 795 003, Manipur, India.
Email: [email protected]

  •  Accepted: 25 February 2011
  •  Published: 31 July 2011

Abstract

Investigation on the chemical constituents including the chemical structure of major components in the water extracts from Strobilanthes flaccidifolius Nees. and Indigofera tinctoria Linn. was done. It was found that the indigo from fresh leaves of S. flaccidifolius Nees.fermented for three days gave the highest amount of indigo. The thin layer chromatography of the crude extract revealed four pigments; the major pigments were of red and blue having Rfvalues of 0.88 and 0.76, respectively. The Rf value, the maximum absorption from UV-Visible spectroscopy and infra red spectrum of blue pigment were the same as those of the standard indigo. Both blue and red pigments are highly soluble in chloroform whereas only red pigment is soluble in methanol.

 

Key words: Comparative study, Strobilanthes flaccidifolius Nees., Indgofera tinctoria Linn., indigo.