International Journal of
Physical Sciences

  • Abbreviation: Int. J. Phys. Sci.
  • Language: English
  • ISSN: 1992-1950
  • DOI: 10.5897/IJPS
  • Start Year: 2006
  • Published Articles: 2557

Full Length Research Paper

Purification and anti-fatigue activity of flavonoids from corn silk

Qing-Lan Hu1*, Li-Jin Zhang1, Yan-Nan Li2, Yong-Jiang Ding3 and Feng-Lin Li4
1Kunming University of Science and Technology, Kunming, Yunnan Province 650224, People’s Republic of China. 2Yunnan Agricultural University, Kunming, Yunnan Province 650201, People’s Republic of China. 3Top Vocational Institute of Information and Technology of Shaoxing, Shaoxing, Zhejiang Province 312000, People’s Republic of China. 4Jilin College of Agricultural Science and Technology, Jilin, Jilin Province 132101, People’s Republic of China.
Email: [email protected]

  •  Accepted: 28 January 2010
  •  Published: 30 April 2010


Flavonoids are major active ingredient in corn silk (CS) and possess various pharmacological activities. In this study, microporous resin adsorption technology was used for obtaining purified flavonoids from corn silk (FCS) and its anti-fatigue activity was evaluated. Eight kinds of macroporous resins with different properties were tested through static adsorption and one macroporous resin labeled as AB-8 was selected. Some important parameters were optimized for most effective enrichment and preparative separation. As follows: 1. for adsorption: sample solution FCS concentration was 2.636 mg/mL; processing volume was 4 BV; flow rate was 2 BV/h; temperature was 25°C; 2. for desorption: elution solvent ethanol-water (90%, v/v) solution was 4BV; flow rate was 1.0 BV/h. Flavonoids with content of 63.15% was obtained with a flavonoid recovery of 94.27% in the purification process. The results showed that AB-8 resin revealed a good ability to purify FCS. Swimming exercise results indicated that FCS had anti-fatigue activity of mice by inhibiting the production of blood lactic acid, retarding the formation of BUN and increasing hepatic glycogen concentration.


Key words: Macroporous resins, purification, anti-fatigue activity, corn silk.