International Journal of
Water Resources and Environmental Engineering

  • Abbreviation: Int. J. Water Res. Environ. Eng.
  • Language: English
  • ISSN: 2141-6613
  • DOI: 10.5897/IJWREE
  • Start Year: 2009
  • Published Articles: 332

Full Length Research Paper

Performance and kinetic evaluation of phenol biodegradation by mixed microbial culture in a batch reactor

Sudipta Dey1*and Somnath Mukherjee2
  1Department of Biotechnology, Heritage Institute of Technology, Anandapur, Chowbaga Road, PO:  Kolkata, PIN: 700107, West Bengal, India. 2Professor, Environmental Engineering Division, Civil Engineering Department, Jadavpur University, Raja S. C. Mallic Road, Kolkata, PIN: 700032, West Bengal, India.
Email: [email protected]

  •  Accepted: 22 April 2010
  •  Published: 31 May 2010

Abstract

 

Mixed microbial culture collected from effluent treatment plant of a coke oven industry has been studied for its phenol biodegrading potential under aerobic condition in a batch reactor. The result showed that, after acclimatization, the culture could biodegrade up to 700 mg/l of phenol. The results showed that specific growth rate of microorganisms and specific substrate degradation rate increased up to 300 mg/l of initial phenol concentration and then started decreasing. The biodegradation kinetics is fitted to different substrate inhibition models by MATLAB 7.1©. Among all models, Haldane model was best fitted (Root Mean Square Error = 0.0067) for phenol degradation. The different biodegradation constants (Ks, Ki, Sm, mmax, YX/S, kd) estimated using these models showed good potential of the mixed microbial culture in phenol biodegradation.

 

Key words: Mixed culture, phenol biodegradation, kinetics, inhibition model.