International Journal of
Water Resources and Environmental Engineering

  • Abbreviation: Int. J. Water Res. Environ. Eng.
  • Language: English
  • ISSN: 2141-6613
  • DOI: 10.5897/IJWREE
  • Start Year: 2009
  • Published Articles: 345

Full Length Research Paper

EROD and PAH detection as biomonitoring tool in Golden Horn-Istanbul Turkey

Sebahat ÅžEKER      
Department of Environmental Engineering, Ardahan University, Ardahan, Turkey.
Email: [email protected]

  •  Accepted: 29 March 2012
  •  Published: 31 May 2012

Abstract

 

This investigation covers studies on the concentrations of Polycyclic Aromatic Hydrocarbons (PAHs) in Golden Horn and their effects on Ethoxyresorufin O-deethylase EROD activities of human hepatocellular carcinoma cell line, HepG2. Water samples were collected from eight different stations around Golden Horn. Concentrations of total water PAHs ranged from 3.8 ng/L to about 259.7 ng/L. CYP1As enzymes were determined based on EROD Activity. A good correlation between total PAHs and EROD activity was observed. The analytical detection and biochemical EROD activity showed that the aquatic ecosystem around Golden Horn contained some hazardous PAHs. Possible sources for these chemicals could be industrial discharge and domestic wastewaters, transportation and shipping activities.  Furthermore this study showed that EROD activity could be used as a bio-monitoring tool for the health effects of PAHs in aquatic ecosystems.

 

Key words: EROD, CYP1As, HepG2, Golden Horn, PAHs.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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