Metronidazole is an active antibacterial that is highly effective against trichomoniasis and protozoan infections. The electrocatalytic properties of the ZnO&CPE electrode were examined using cyclic voltammetry (CV), EIS. Zinc oxide nanoparticles formed on graphite carbon sheets under optimized experimental conditions showed excellent electrocatalytic performance for the reduction of trace amounts of metronidazole. The surface morphology of the fabricated electrode was characterized by scanning electron microscopy, the chemical composition of ZnO&CPE was investigated by DRX. The calibration curve was constructed by differential pulse voltammetry method in the concentration range of 0.8-100 µmol L-1, with detection and quantification limit of metronidazole equal to 1.018× 10-7 M and 3.395× 10-7 M, respectively. The proposed method has been successfully tested for the detection of trace amounts of metronidazole in real samples such as urine and serum. The proposed method is also simple, fast and inexpensive. It has a good recovery rate, good selectivity and high sensitivity.

Keywords: Metronidazole, antibacterial, carbon graphite, ZnO&CPE, electrocatalytic detection,