Agricultural practices void of chemicals is the most current approach in sustainable agricultural system. Mutants of Glomus versiforme serves as an excellent source of bioactive compounds in the control of plant pathogens. UV mutagenesis was found to be one of the novel strategy for developing Glomus versiforme mutants with enhanced inhibitory activity. Secondary metabolites of Glomus versiforme (wild and mutant) were extracted, screened for biofungicidal constituents and were assayed against the fungal pathogen.Analysis of fungitoxic constituents tested positive for Alkaloids, Phenols, Flavonoids, Saponins, Tannins, Phlobatannins and Steroids, While Terpenoids and Cardiac glycosides tested negative.The results of antimicrobial susceptibility using the crude extracts from wild and mutant strains Glomus versiforme at different concentrations of 200mg/mL, 100mg/mL, 50mg/mL and 25mg/mL showed that Gv8 highly inhibits radial mycelia growth of the pathogen.The order of susceptibility follows Gv8>Gv4>Gvw>Gv12. Crude extract from Gv8 has the lowest MIC and MFC of 30.0 mg/mL and 43.3 mg/mL respectively followed by Gv4 with MIC and MFC of 31.3 mg/mL and 54.0 mg/mL. MIC and MFC values of Gv12 is the highest with 72.7 mg/mL and 92.0 mg/mL respectively. Generally, the MIC and MFC results agrees with the order of antimicrobial susceptibility in that, Gv8

Keywords: Phytochemicals, mutants, Glomus versiforme, mutagenesis, Erysiphe flexuosa