African Journal of
Bacteriology Research

  • Abbreviation: J. Bacteriol. Res.
  • Language: English
  • ISSN: 2006-9871
  • DOI: 10.5897/JBR
  • Start Year: 2009
  • Published Articles: 106

Full Length Research Paper

Multidrug-resistant tuberculosis in HIV-negative patients in Lagos, Nigeria

Kunle-Ope Chioma N.
  • Kunle-Ope Chioma N.
  • Centre for Tuberculosis Research, Nigerian Institute of Medical Research, Lagos, Nigeria.
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Adeleye Isaac A.
  • Adeleye Isaac A.
  • Department of Microbiology, University of Lagos Akoka, Lagos, Nigeria.
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Iwalokun Bamidele O.
  • Iwalokun Bamidele O.
  • Molecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Lagos, Nigeria.
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Igbasi Uche T.
  • Igbasi Uche T.
  • Centre for Infectious Disease Research, Nigerian Institute of Medical Research, Lagos, Nigeria.
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Onyejepu Nneka
  • Onyejepu Nneka
  • Centre for Tuberculosis Research, Nigerian Institute of Medical Research, Lagos, Nigeria.
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Ndubuisi Ifeanyi T.
  • Ndubuisi Ifeanyi T.
  • Centre for Tuberculosis Research, Nigerian Institute of Medical Research, Lagos, Nigeria.
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Tochukwu Nwanneka E.
  • Tochukwu Nwanneka E.
  • Centre for Tuberculosis Research, Nigerian Institute of Medical Research, Lagos, Nigeria.
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Adedeji Abimbola M.
  • Adedeji Abimbola M.
  • Monitoring and Evaluation Unit, Nigerian Institute of Medical Research, Lagos, Nigeria.
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Onwujekwe Daniel I.
  • Onwujekwe Daniel I.
  • Clinical Science Department, Nigerian Institute of Medical Research, Lagos, Nigeria.
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Ezechi Oliver C.
  • Ezechi Oliver C.
  • Clinical Science Department, Nigerian Institute of Medical Research, Lagos, Nigeria.
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Odunukwe Nkiruka N.
  • Odunukwe Nkiruka N.
  • Clinical Science Department, Nigerian Institute of Medical Research, Lagos, Nigeria.
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Iyke-Azike Obioma G.
  • Iyke-Azike Obioma G.
  • Centre for Tuberculosis Research, Nigerian Institute of Medical Research, Lagos, Nigeria.
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Onubogu Catherine C.
  • Onubogu Catherine C.
  • Centre for Tuberculosis Research, Nigerian Institute of Medical Research, Lagos, Nigeria.
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  •  Received: 14 July 2020
  •  Accepted: 30 October 2020
  •  Published: 30 November 2020

Abstract

Multidrug-resistant tuberculosis (MDR-TB) is a big challenge to the tuberculosis control programmes worldwide. The aim of this work is to determine the rate of MDR-TB in HIV-negative patients with signs and symptoms of pulmonary tuberculosis in Lagos State. 891 adult HIV-negative patients enrolled for the study and tested for acid-fast bacilli by culture; resistance to first-line anti-tuberculosis drugs was performed (isoniazid – 0.2 µg/ml, rifampicin – 40 µg/ml, and ethambutol – 2 µg/ml) by culture method on Lowenstein Jensen (LJ) medium using proportion method. Of the 819 HIV-negative patients, 47.7% were positive for acid-fast bacilli by culture, and 78% of the culture positive were found to be Mycobacterium tuberculosis complex (MTBC). DST analysis on MTBC isolates showed that resistance to only one drug was found in forty-two (13.5%) patients, dual resistance in eighty-seven (28.3%) patients while triple resistance in 45 (14.6%). A total of 117 (38%) MDR-TB cases were recorded in this study. Sex, age, tuberculosis contact, smoking and alcohol consumption were not significantly associated with MDR-TB, P>0.05. However, previous history of tuberculosis was significantly associated with MDR-TB, P = 0.000. This study concluded that there was high MDR-TB rate (38%) among HIV-negative patients and MDR-TB is associated with previous history of tuberculosis. The findings emphasize the importance of continuing the systematic surveillance of drug resistance for prompt diagnosis and iniatiation of treatment.

 

Key words: Lagos, HIV-negative, Directly Observed Treatment Short-course (DOTS), Multidrug-resistant tuberculosis (MDR-TB).

Abbreviation

MDR-TB, Multidrug-resistant tuberculosis; TB, Tuberculosis; HIV, Human Immunodeficiency Virus; DOTS, Directly Observed Treatment Short-course; AFB, Acid-fast bacilli; LJM: Lowenstein Jensen Medium; ZN, Ziehl Neelsen; MTB, Mycobacterium tuberculosis; MTBC, Mycobacterium tuberculosis complex; DST, Drug Susceptibility Testing; WHO, World Health Organization; NIMR, Nigerian Institute of Medical Research; IDH, Infectious Disease Hospital; CTBR, Centre for Tuberculosis Research.