African Journal of
Bacteriology Research

  • Abbreviation: J. Bacteriol. Res.
  • Language: English
  • ISSN: 2006-9871
  • DOI: 10.5897/JBR
  • Start Year: 2009
  • Published Articles: 103

Full Length Research Paper

Prevalence and characterization of NDM-1 and OXA-48 carbapenemase gene harboring Enterobacteriaceae in a tertiary care hospital, South India

Radha Srinivasan*
  • Radha Srinivasan*
  • Department of Microbiology. JIPMER, Puducherry-605 006, India.
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Kalaiarasan Ellappan
  • Kalaiarasan Ellappan
  • Department of Microbiology. JIPMER, Puducherry-605 006, India.
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Harish Belgode Narasimha
  • Harish Belgode Narasimha
  • Department of Microbiology. JIPMER, Puducherry-605 006, India.
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  •  Received: 17 June 2015
  •  Accepted: 12 August 2015
  •  Published: 30 October 2015

Abstract

The occurrence of carbapenem resistance among Enterobacteriaceae has reached critical levels worldwide. The present study was carried out to screen for carbapenem resistance among Enterobacteriaceae isolates from blood, surgical wound and tracheal samples in different wards and intensive care units between the period of 2010 through 2012 in a teaching hospital attached to JIPMER, south India. A total number of 425 meropenem-resistant isolates belonging to Enterobacteriaceae were included. Genes encoding β-lactamases (blaNDM-1 and blaOXA-48) and influx of β-lactams through the major porin OmpK36 were characterized. Multiplex PCR amplification assays indicated that out of 425 meropenem- resistant isolates, 75 and 187 isolates harbored blaNDM-1 with and without OmpK36, respectively. One and five Klebsiella pneumoniae isolates harbored blaOXA-48 with presence and absence of OmpK36, respectively. Transferability of the plasmids was tested by conjugation assays and it was found that 110 transconjugants carried blaNDM-1. blaOXA-48 gene was not transferable by conjugation analysis. The study highlights the emergence of blaNDM-1 and blaOXA-48 genes in this center.
 
Key words: Enterobacteriaceae; blaNDM-1; blaOXA-48; OmpK36; conjugation.