African Journal of
Bacteriology Research

  • Abbreviation: J. Bacteriol. Res.
  • Language: English
  • ISSN: 2006-9871
  • DOI: 10.5897/JBR
  • Start Year: 2009
  • Published Articles: 103

Full Length Research Paper

Detection of biofilm formation of a collection of fifty strains of Staphylococcus aureus isolated in Algeria at the University Hospital of Tlemcen

GHELLAI Lotfi*,
  • GHELLAI Lotfi*,
  • Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE), Department of Biology, University of Tlemcen, 13000 Tlemcen, Algeria.
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HASSAINE Hafida
  • HASSAINE Hafida
  • Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE), Department of Biology, University of Tlemcen, 13000 Tlemcen, Algeria.
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KLOUCHE Nihel
  • KLOUCHE Nihel
  • Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE), Department of Biology, University of Tlemcen, 13000 Tlemcen, Algeria.
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KHADIR Abdelmonaim
  • KHADIR Abdelmonaim
  • Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE), Department of Biology, University of Tlemcen, 13000 Tlemcen, Algeria.
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AISSAOUI Nadia
  • AISSAOUI Nadia
  • Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE), Department of Biology, University of Tlemcen, 13000 Tlemcen, Algeria.
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NAS Fatima
  • NAS Fatima
  • Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE), Department of Biology, University of Tlemcen, 13000 Tlemcen, Algeria.
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ZINGG Walter
  • ZINGG Walter
  • Service de Prévention et de Contrôle de l’Infection. Hôpitaux Universitaires de Genève (HUG) Suisse.
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  •  Received: 30 September 2013
  •  Accepted: 06 February 2014
  •  Published: 28 February 2014

Abstract

The burden of disease caused by Staphylococcus aureus continues to grow; this organism has the ability to form biofilm and it is also a frequent cause of medical device and implant-related infections. The objective of this study was to evaluate the biofilm-forming ability of a collection of clinical isolates of S. aureus. In a total of 240 Staphylococcus spp. isolated from catheters, retrieved at five services (neonatology, internal medicine, pneumology, pediatric and neurology), only 50 (20.83%) strains were identified by conventional microbiological methods as S. aureus species; these strains were screened by microtiter plate assay for detection of biofilm formation. Of the 50 clinical isolates, 16 (32%) were non adherent, 20(40%) weakly, 10 (20%) moderately and 4(8%) strongly adherent. The quantitative method of microtiter plate can be involved as a simple, rapid, inexpensive and reproducible assay to assess biofilm formation which is further an important feature of pathogenecity of S. aureus in the clinical setting.

Key words: Microbial biofilm, Staphylococcus aureus, catheter, microtiter plate assay.