African Journal of
Bacteriology Research

  • Abbreviation: J. Bacteriol. Res.
  • Language: English
  • ISSN: 2006-9871
  • DOI: 10.5897/JBR
  • Start Year: 2009
  • Published Articles: 109

Full Length Research Paper

Use of real time polymerase chain reaction (PCR) and histopathological changes for detection of the Toxoplasma gondii parasite in male rats (experimental study)

Ghiadaa Abass Jassem
  • Ghiadaa Abass Jassem
  • College of Veterinary Medicine, AL-Qadisiyia University, Iraq.
  • Google Scholar

  •  Received: 24 September 2014
  •  Accepted: 23 December 2014
  •  Published: 31 January 2015



Abdoli A, Dalimi A, Movahedin M (2012). Impaired reproductive function of male rats infected with Toxoplasma gondii. Inst. Andrologia . 44:679-687
Afshari F, Imani A, Asl S, Farhang H, Ghasempour K, Ezzatzadeh A, Ainechi N (2013). Evaluation of Testosterone and Alkaline Phosphatase Activity Changes in Epidydimis of Toxoplasma gondii Infected Rats. Int. J. Women's Health Reprod. Sci. 1(2):1-8.
Ahmad M, Maqbool A, Mahmood-ul-Hassan M, Anjum A (2012). Prevalence of Toxoplasma gondii antibodies in human beings and commensally rodents trapped from Lahore, Pakistan. J. Anim. Plant Sci. 22(1):51-53.
Alkennay E, Hassan S (2010). Pathological study on some tissues of rat experimentally infected with Toxoplasma gondii . J. Edu. Sci. 23(3):8-17.
Al-Khaffaf F, Abdullah B (2005). Isolation of Toxoplasma gondii Parasite from Placental Tissue of Aborted Women in Nenavah Governerate. Alrafedian J. Sci. 6(16):93-99.
Al-taie A, Abdulla S (2008). Toxoplasma gondii: Experimental infection of Isolated local strain in Sulaimani Province Iraqi. J. Med. Sci. 6(3):60-71.
Alves M, Silva A, Bianchi L, Araujo E, Sant'Ana D (2011). Toxoplasma gondii induces death of gastric myenteric neurons in Rats. Int. J. Morphol. 29(1):293-298.
Amerizadeh A, Khoo B, The A, Golkar M, Karim IZ, Osman S, Yunus M, Noordin R (2013). Identification and real-time expression analysis of selected Toxoplasma gondii in-vivo induced antigens recognized by IgG and IgM in sera of acute toxoplasmosis patients, BMC Infect. Dis.13(287):1-12.
Dalimi A, Abdoli A (2013). Toxoplasma gondii and Male Reproduction Impairment: A new aspect of Toxoplasmosis Research, Jundishapur J. Microbiol. 6(8):e7184.
Donovan J, Proctor A, Gutierrez J, Worrell S, Nally J, Marques P, Brady C, McElroy M, Sammin D, Buxton D, Maley S, Bassett H and Markey B (2012). Distribution of lesions in fetal brains following experimental infection of pregnant sheep with Toxoplasma gondii, Vet. Pathol. 49(3):462-469.
Dubey J, Jones J (2008).Toxoplasma gondii infection in humans and animals in the United States, Int. J. Parasitol. 38:1257-1278.
Dubey J, Lindsay D (2006). Neosporosis, Toxoplasmosis, and Sarcocystosis in ruminants. Vet. Clin. Food Anim. 22:645-671.
Figueiredo J, Silva D, Cabral D, Mineo J (2001). Seroprevalence of Toxoplasma gondii Infection in goats by the indirect haemagglutination, Immunofluorescence and immunoenzymatic tests in the region of Uberlândia, Brazil MemInst Oswaldo Cruz, Rio de.Janeiro. 96(5):687-692.
Gheoca D, Haranglavean A, Gheoca V (2009). Aspect of Toxoplasma Toxoplasma-like parasitism in small mammal species in Transylvania and there role in Toxoplasma dissemination,VasileGoldis, Seria Ştiinele Vieţii . 19(1):69-73.
Gunel T, Kalelioglu I, Ermis H, Has R, Aydinli K (2012). Large scale pre-diagnosis of Toxoplasma gondii DNA genotyping by real-time PCR on amniotic fluid, Biotechnol. Biotechnol. 26(2):2913-2915.
Hill D, Dubey J (2002). Toxoplasma gondii: transmission, diagnosis and prevention,Clin . Microbiol. Infect. 8:634-640.
Lin M, Chen T, Kuo T, Tseng C, Tseng C (2000). Real-Time PCR for Quantitative Detection of Toxoplasma gondii . J. Clin. Microbiol . 38(11):4121-4125.
Lopes W, Santos T, Luvizotto M, Sakamoto C, Oliveira G, Costa A (2011). Histopathology of the reproductive system of male sheep experimentally infected with Toxoplasma gondii,Parasitol. Res. 109:405-409.
Mei-Hui L, Tse-Ching C, Tseng Tong K, Chung T, Ching – Ping T (2000). Real- Time PCR for Quantitative Detection of T. gondii . J. Clin. Microbiol. 38(11):4121-4125.
Mesquita R, Ziegler A, Hiramoto R, Vidal J, Chioccola V (2010). Real-time quantitative PCR in cerebral toxoplasmosis diagnosis of Brazilian human immunodeficiency virus-infected patients, J. Med. Microbial. 59:641-647.
Miller D, Davis J, Rosa R, Diaz M, Perez E (2000). Utility of Tissue Culture for Detection of Toxoplasma gondii in Vitreous Humor of Patients Diagnosed with Toxoplasmic Retinochoroiditis. J. Clin. Microbiol. 38(10):3840-3842.
Moreno B, Fernández E, Villa A, Navarro A, Cerrillo J, Mora L (2012). Occurrence of Neospora cranium and Toxoplasma gondii infections in ovine and caprine abortions. Vet. Parasitol. pp. 1-7.
Oyedeji K, Bolarinwa A, Oladosu I (2013). Effect Of Isolated Lupone Constitunet of Portulacaoleracea on Reproductive parameters in male rats. Int. J. Pharm. Sci. 5(2):684-690.
Salibay C, Claveria F (2006). Toxoplasma gondii infection in Philippines Rattu sspp. Confirmed through bioassay in Musmusculus. Vet. Arhiv. 76(4):351-361.
Scarpelli L, Lopes D, Migani M, Bresciani K, Costa A (2009). Toxoplasma gondii in experimentally infected Bosaurus and Bosindicus semen and tissues, Pesq. Vet. Bras. 29(1):59-64.
Shi-guo L, Hai-zhu Z, Xin L, Zhe Z, Bin H (2006). Dynamic observation of polypide in semen and blood of rabbits infected with Toxoplasma tachyzoites. Chin. Med. J. 119(8):701-704.
Terpsidis K, Papazahariadou M, Taitzoglou I, Papaioannou N, Georgiadis M, Theodoridis I (2009).Toxoplasma gondii Reproductive parameters in experimentally infected male rats. Exp. Parasitol. 121:238-241.