Journal of
Computational Biology and Bioinformatics Research

  • Abbreviation: J. Comput. Biol. Bioinform. Res
  • Language: English
  • ISSN: 2141-2227
  • DOI: 10.5897/JCBBR
  • Start Year: 2009
  • Published Articles: 39

Full Length Research Paper

An insight into blood clotting disorders in humans

Parul Johri1*, Sagar Nagare1, Kakumani Venkateswara Swamy2 and Chitta Suresh Kumar2
1Department of Biotechnology and Bioinformatics, Dr. D. Y. Patil University, Navi Mumbai, India. 2Bioinformatics Facility, Department Biochemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India.
Email: [email protected]

  •  Accepted: 19 November 2010
  •  Published: 31 January 2011


The structure of thrombin protein Oncorhynchus mykiss had not yet been resolved by NMR or X-ray diffraction method. In the present work, we have modeled the structure of protein using Modeller 9v1, taking human thrombin protein as the template. Furthermore, the antithrombin protein was docked with the best model generated by R-dock and Z-dock module of INSIGHT II and the nine potential amino acids involved in anchoring of antithrombin with thrombin were identified as: Leu3, Trp27, Asn120, Glu123, Pro133, Ile148, Ser152, Gly204 and Phe215, respectively. The study also showed surface complimentarily between O. mykiss thrombin and antithrombin which can be helpful for designing new antithrombin molecules for the treatment of blood clotting disorders in humans.


Key words: Antithrombin protein, homology modeling, r-dock, thrombin, z-dock.