Loss of heterozygosity (LOH) of deleted in colorectal cancer (DCC) gene, a putative tumour suppressor gene has been implicated in development of various cancers. Here we aim to study the frequency of LOH of DCC gene in colorectal cancer (CRC) patients of Kashmir and further to assess any association with etio-pathological factors. Genomic DNA from 80 confirmed CRC tissue samples as well as adjacent normal tissues were used in this study. LOH of DCC gene was detected using two intronic microsatellite markers, D18S8-M2 and variable number tandem repeat (VNTR) by restriction fragment length polymorphism (RFLP) and amplified fragment length polymorphism (AFLP) respectively. LOH in DCC was observed in 26 of 80 (32.5%) at VNTR and 19 of 80 (23.75%) at D18S8-M2 region in CRC cases. The combined frequency of LOH of DCCgene of two markers aggregated to 56.25 % (45 of 80) of CRC cases. In this study it is found that LOH has a frequency of 56% in patients with CRC and is highly frequent in patients with higher stage/grade in CRC.
Key words: Colorectal cancer, restriction fragment length polymorphism, loss of heterozygosity, microsatellite markers.
ASR, Age standardized rate, DCC, deleted in colorectal cancers; VNTR,variable number tandem repeat; MSI, microsatellite instability; RFLP, restriction fragment length polymorphism.
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