Journal of
General and Molecular Virology

  • Abbreviation: J. Gen. Mol. Virol.
  • Language: English
  • ISSN: 2141-6648
  • DOI: 10.5897/JGMV
  • Start Year: 2009
  • Published Articles: 37

Full Length Research Paper

Molecular characterization of genetic diversity of tomato (Solanum lycopersicum L.) begomovirus in Togo

Assion Setu MIVEDOR
  • Assion Setu MIVEDOR
  • Laboratory of Plant Virology and Biotechnology (LVBV), Ecole Supérieure d'Agronomie (ESA) / University of Lome, Togo.
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Kodjovi Atasse DANSOU-KODJO
  • Kodjovi Atasse DANSOU-KODJO
  • Laboratory of Plant Virology and Biotechnology (LVBV), Ecole Supérieure d'Agronomie (ESA) / University of Lome, Togo.
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Kossikouma Djodji ADJATA
  • Kossikouma Djodji ADJATA
  • Laboratory of Plant Virology and Biotechnology (LVBV), Ecole Supérieure d'Agronomie (ESA) / University of Lome, Togo.
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Jerome DUCLERCQ
  • Jerome DUCLERCQ
  • Laboratory of Agroecology, Ecophysiology and Integrative Biology (AEB), Unit EDYSAN FRE 3498 CNRS / University of Picardie Jules Verne, 33 rue St Leu 80039 Amiens, France.
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Yawovi Mawuena Dieudonne GUMEDZOE
  • Yawovi Mawuena Dieudonne GUMEDZOE
  • Laboratory of Plant Virology and Biotechnology (LVBV), Ecole Supérieure d'Agronomie (ESA) / University of Lome, Togo.
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  •  Received: 25 February 2017
  •  Accepted: 09 November 2017
  •  Published: 30 November 2017

Abstract

Geminiviruses, in particular the members of the genus begomovirus, are considered to be a major phytosanitary problem for tomato crops production in the world. They are responsible for yield losses of up to 20 to 100% and Togo is not spared from this situation. The present study aimed to investigate the genetic diversity of the begomoviruses affecting tomato crops production in Togo and their relationship with other begomoviruses. To achieve these objectives, 307 samples of tomato leaves and wild plant species with typical virus symptoms were collected in the Maritime, Plateau, Central, Kara and Savannah regions and subjected to polymerase chain reaction (PCR) analysis. The results revealed the presence of begomovirus in 25.40% of the analyzed samples. The PCR products obtained were subjected to direct sequencing. Phylogenetic analysis of the sequences of the different regions of the DNA-A of the begomovirus identified in this study with that of other begomoviruses showed a nucleotide identity of 96% respectively to Tomato leaf curl Togo virus-Fontem, Tomato Leaf Curl Togo Virus, and Ageratum leaf curl Cameroon Alphasatellite; 98% respectively to Tomato leaf curl Nigeria virus, Ageratum leaf curl Cameroon virus, Tomato leaf curl Cameroon virus-Fontem, Ageratum leaf curl Cameroon virus and 99% respectively to Tomato leaf curl Kumasi virus, Pepper yellow vein Mali virus Bazegahot, and Pepper yellow vein Mali virus-Ouaga. These results suggest a high degree of genetic diversity of tomato begomoviruses identified in Togo.
 
Key words: Begomoviruses, genetic diversity, phylogenetic relationships, tomato, wild plants.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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