Journal of
General and Molecular Virology

  • Abbreviation: J. Gen. Mol. Virol.
  • Language: English
  • ISSN: 2141-6648
  • DOI: 10.5897/JGMV
  • Start Year: 2009
  • Published Articles: 36

Full Length Research Paper

A two-end point analysis reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of maize chlorotic mottle virus (MCMV)

Francis M. Mwatuni
  • Francis M. Mwatuni
  • Kenya Plant Health Inspectorate Service, P. O. Box 49421-00100 Nairobi, Kenya.
  • Google Scholar
Margaret Peg Radinbaugh
  • Margaret Peg Radinbaugh
  • Department of Plant Pathology, Ohio State University, Wooster 44691OH, USA.
  • Google Scholar
Sally Miller
  • Sally Miller
  • Department of Plant Pathology, Ohio State University, Wooster 44691OH, USA.
  • Google Scholar
Xing Ma
  • Xing Ma
  • Department of Plant Pathology, Ohio State University, Wooster 44691OH, USA.
  • Google Scholar
Aggrey B. Nyende
  • Aggrey B. Nyende
  • Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00100 Nairobi, Kenya.
  • Google Scholar
L. M. Suresh
  • L. M. Suresh
  • International Maize and Wheat Improvement Center (CIMMYT), P. O. Box 1041 – 00621 Nairobi, Kenya.
  • Google Scholar
Ivan Obare
  • Ivan Obare
  • Kenya Plant Health Inspectorate Service, P. O. Box 49421-00100 Nairobi, Kenya.
  • Google Scholar


  •  Received: 22 April 2020
  •  Accepted: 09 October 2020
  •  Published: 30 November 2020

References

Adams IP, Harju VA, Hodges T, Hany U, Skelton A, Rai S, Deka MK, Smith J, Fox A, Uzayisenga B, Ngaboyisonga C, Uwumukiza B, Rutikanga A, Rutherford M, Ricthis B, Phiri N, Boonham N (2014). First report of maize lethal necrosis disease in Rwanda. New Diseases Reports 29:22.
Crossref

 

Boddupalli P, Suresh LM, Mwatuni F, Beyene Y, Makumbi D, Gowda M, Olsen M, Hodson D, Worku M, Mezzalama M, Molnar T (2020). Maize lethal necrosis (MLN): Efforts toward containing the spread and impact of a devastating transboundary disease in sub-Saharan Africa. Virus Research 282:197943.
Crossref

 
 

Braidwood L, Quito-Avila DF, Cabanas D, Bressan A, Wangai A, Baulcombe DC (2018). Maize chlorotic mottle virus exhibits low divergence between differentiated regional sub-populations. Scientific Reports 8(1):1-9.
Crossref

 
 

Burundi Institute of Agronomic Sciences (ISABU) (2017). MLN surveillance report 2017. Burundi Institute of Agronomic. Sciences (ISABU).

 
 

Castillo J, Hebert TT (1974). A new virus disease of maize in Peru. Fitopatologia 9(2):79-84.

 
 

Chen L, Zhiyuan J, Dongmei L, Xingliang L, Zihao X, Congliang D, Tao Z, Zaifeng F (2016). One-step reverse transcription loop-mediated isothermal amplification for the detection of Maize chlorotic mottle virus in maize. Journal of Virological Methods 240:49-53.
Crossref

 
 

CIMMYT MLN Epidemiology Report (2019). Maize Lethal Necrosis Diagnostics and Prevention of Seed Transmission. Available at: View

 
 

De Groote H, Oloo F, Tongruksawattana S, Biswanath D (2016). Community-survey based assessment of the geographic distribution and impact of maize lethal necrosis (MLN) disease in Kenya. Crop Protection 82:30-35.
Crossref

 
 

EIAR MLN surveillance Report (2019). Ethiopian Institute of Agricultural research (EIAR). Available at: 

View

 
 

Goujon M, McWilliam H, Li W, Valentin F, Squizzato S, Paern J, Lopez R (2010). A new bioinformatics analysis tools framework at EMBL-EBI. Nucleic Acids Research 38(suppl_2):W695-W699.
Crossref

 
 

Hsieh K, Mage PL, Csordas AT, Eisenstein M, Soh HT (2014). Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP). Chemical Communications 50(28):3747-3749.
Crossref

 
 

Ignatov KB, Ekaterina VB, Arkady FF, Konstantin AB, Tatiana VK, Vladimir MK (2014). A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification. Biological Techniques 57:81-87.
Crossref

 
 

Jensen SG, Wysong DS, Ball EM, Higley PM (1991). Seed transmission of maize chlorotic mottle virus. Plant Diseases 75:497-498.
Crossref

 
 

Kagoda F, Gidoi R, Isabirye BE (2016). Status of maize lethal necrosis in eastern Uganda. African Journal Agricultural Research 11:652-660.
Crossref

 
 

Lukanda M, Owati A, Ogunsanya P, Valimunzigha K, Katsongo K (2014). First report of maize chlorotic mottle virus infecting maize in the Democratic Republic of the Congo. Plant Diseases 98:1448-1449.
Crossref

 
 

Mackay IM, Arden KE, Nitsche A (2002). Real-time PCR in virology. Nucleic Acids Research 30:1292-1305.
Crossref

 
 

Mahuku G, Lockhart BE, Wanjala B, Jones MW, Kimunye JN, Stewart LR, Cassone BJ, Sevgan S, Nyasani JO, Kusia E, Kumar PL (2015b). Maize lethal necrosis (MLN), an emerging threat to maize-based food security in sub-Saharan Africa. Phytopathology 105(7):956-965.
Crossref

 
 

Mahuku G, Wangai A, Sadessa K, Teklewold A, Wegary D, Ayalneh D, Adams I, Smith J, Bottomley E, Bryce S, Braidwood L (2015a). First report of maize chlorotic mottle virus and maize lethal necrosis on maize in Ethiopia. Plant Diseases 99:1870-1874.
Crossref

 
 

Marchand JL, Peterschmitt M, Reynaud B, Dintinger J (1995). Maize streak virus, maize stripe virus and maize mosaic virus in the tropics (Africa and islands in the Indian Ocean). Agriculture and Development Special Issue. pp. 55-69. Available at: 

View

 
 

Ministry of Agriculture Kenya (2012). Ministry of Agriculture, Livestock and Development Biannual report.

 
 

National Cereal Crops Research (NaCCRI) (2017). MLN Surveillance reports 2017. National Cereal Crops Research Institute (NaCRRI, Uganda). Available at: 

View

 
 

National Cereal Crops Research (NaCCRI) (2019). MLN Surveillance reports 2018. National Cereal Crops Research Institute (NaCRRI, Uganda). Available at: 

View

 
 

Niblett CL, Claflin LE (1978). Corn lethal necrosis a new virus disease of corn in Kansas. Plant Disease 62:15-19.

 
 

Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino, N, Hase T (2000). Loop-mediated isothermal amplification of DNA. Nucleic Acids Research 28(12):e63-e63.
Crossref

 
 

Nutter RC, Scheets K, Panganiban L, Lommel SA (1989). The complete nucleotide sequence of maize chlorotic mottle virus. Nucleic Acids Research 17:3163-3177.
Crossref

 
 

Nyvall R (1999). Field crop diseases (Third Edition). Iowa State University Press, Ames, Iowa. ISBN-13: 978-0813820798.

 
 

Parida MM, Guillermo P, Inoue S, Hasebe F, Morita K (2004). Real-time reverse transcription-loop-mediated isothermal amplification for rapid
Crossref

 
 

detection of West Nile virus. Journal of Clinical Microbiology 42:257-263.

 
 

Parida MM, Santosh SR, Dash PK, Tripathi NK, Saxena P, Ambuj S, Sahini AK, Laksman PV, Rao MK (2006). Development and evaluation of reverse transcription loop mediated isothermal amplification assay for rapid and real-time detection of Japanese encaphalitis virus. Journal of Clinical Microbiology 44(11):4172-4178.
Crossref

 
 

Quito-Avila DF, Alvarez RA, Mendoza AA (2016). Occurrence of maize lethal necrosis in Ecuador. A disease without boundaries? European Journal Plant Pathology 146:705-710.
Crossref

 
 

Riley M, Williamson MR, Maloy O (2002). Plant disease diagnosis. The Plant Health Instructor. 
Crossref

 
 

Scheets K (2000). Maize chlorotic mottle machlomovirus expresses its coat protein from a 1•47-kb subgenomic RNA and makes a 0•34-kb subgenomic RNA. Virology 267:90-101.
Crossref

 
 

Scheets K (2004). Maize chlorotic mottle virus; Viruses and Virus Diseases of Poaceae (Gramineae). H. Lapierre and P. A. Signoret, eds. Institut National de la Rocherche Agronomique, Paris, France. pp. 642-644.

 
 

Shan C, Zhou Y, Li X, Yang S, Shao W (2017). Comparison and application of RT-LAMP and RT-PCR methods for MCMV detection. Guangdong Agricultural Sciences 7:14.

 
 

Soliman H, Ei-Matbouli M (2006). Reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of viral hemorrhagic septicaemia virus (VHS). Veterinary Microbiology 114:205-213.
Crossref

 
 

Uyemoto JK (1983). Biology and control of maize chlorotic mottle virus. Plant Disease 67:7-10.
Crossref

 
 

Uyemoto JK, Bockelman DL, Claflin LE (1980). Severe outbreak of corn lethal necrosis disease in Kansas. Plant Disease 64:99-100.
Crossref

 
 

Wang CY, Zhang QF, Gao YZ, Zhou XP, Ji G, Huang XJ (2015). Insight into the three-dimensional structure of maize chlorotic mottle virus revealed by Cryo-EM single particle analysis. Virology 485:171-178.
Crossref

 
 

Wangai AW, Redinbaugh MG, Kinyua ZM, Mahuku G, Scheets K, Jeffers D (2012). First report of Maize chlorotic mottle virus and maize lethal necrosis in Kenya. Plant Disease 96(10):1582-1583.
Crossref

 
 

Wong YP, Othman S, Lau YL, Radu S, Chee HY (2017). Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms Journal of Applied Microbiology 124:626-643.
Crossref

 
 

Xu Y, Liu Y, Qiu Z, Zheng W (2017). Reverse Transcription-loop-mediated Isothermal Amplification for Detection of Maize chlorotic mottle virus. Agricultural Science and Technology 1:123-126.

 
 

Zhanmin L, Xueying X, Cuiyun Y, Junyi H (2015). Colourimetric detection of Maize chlorotic mottle virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynapthol blue dye. Royal Society of Chemistry Advances 6:73-78.
Crossref