Journal of
Infectious Diseases and Immunity

  • Abbreviation: J. Infect. Dis. Immun.
  • Language: English
  • ISSN: 2141-2375
  • DOI: 10.5897/JIDI
  • Start Year: 2009
  • Published Articles: 87

Full Length Research Paper

Characterization of the influence of Mansonella perstans co-infection on immunity

Codjo Laurent Azonvide
  • Codjo Laurent Azonvide
  • Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cellular Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
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Tomabu Adjobimey
  • Tomabu Adjobimey
  • Biochemistry and Molecular Biology Unit, Department of Biochemistry and Cellular Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
  • Google Scholar
Haziz Sina
  • Haziz Sina
  • Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cellular Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
  • Google Scholar
Lamine Baba-Moussa
  • Lamine Baba-Moussa
  • Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cellular Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
  • Google Scholar


  •  Received: 09 January 2022
  •  Accepted: 05 May 2022
  •  Published: 31 May 2022

Abstract

The study aimed to characterize the influence of Mansonella perstans co-infection on immunity. This research was carried out in November, 2021 in the neighboring villages of Saint Camille de Davougon in the Abomey Commune. Blood samples were collected from both genders of all ages. Buruli ulcer was diagnosed by polymerase chain reaction (PCR), using specific IS2404 repeat sequence for Mycobacterium ulcerans. Microfilaria detection was done with the help of the counting chamber technique. Using 100 white blood cells, the proportion of eosinophils was determined. Minividas® Biomerieux was used to quantify the IgE titers in the patients' serum using an immuno-enzymatic sandwich enzyme linked immunosorbent assay (ELISA). This study shows an overall co-infection of Buruli ulcers with M. perstans as 23.86%. In the age group of fewer than 16 years, the co-infection prevalence was 28.57% (n=6), while in patients of age greater than 16, the co-infection prevalence was 71.42% (n=15). The Buruli ulcer patients co-infected with M. perstans had the highest median eosinophil level, whereas the control group had the lowest. The lowest median total IgE titer was observed in the control, while the maximum was observed in the Buruli ulcer patients co-infected with M. perstans. This study concludes that Buruli ulcer co-infection with M. perstans prevails in some rural villages in Benin. Therefore, this pathogen must be considered in the treatment of Buruli ulcer. The findings imply that IgE could be used to assess the patient's immunological response during Buruli ulcer and M. perstans.

Key words: Mansonella perstans, co-infection, immunity, Buruli ulcer.