October 2010

Molecular characterization of antigens extracted from hydatid cysts of human and other intermediate hosts

This research includes a study of hydatid cysts Echinococcus granulosus (larval stage on the molecular level, where 7 samples of hydatid cysts were collected from parasite intermediate hosts " Human (liver, spleen, lung) and liver of sheep, goat, cattle and buffaloes”. DNA was extracted from germinal layer cells of hydatid cysts which were isolated shortly or preserved for various...

Author(s): Mohammed M. A. Al-Fayadh, Maani N. Al-Shemari and Ihsan E. Al-Saimary

October 2010

Screening of Aspergillus flavus and Aspergillus fumigatus strains for extracellular protease enzyme production

The fungal isolates identified as Aspergillus fumigatus and Aspergillus flavus isolated from a local rice husk dumpsite were screened for protease enzyme production. High yields of protease enzyme were obtained by both fungi after 144 h with concentrations of 0.96 µg/ml/min for A. flavus and 0.84 µg/ml/min for A. fumigatus. Optimum temperature for the...

Author(s): Oyeleke, S. B., Egwim, E. C. and Auta, S. H.

October 2010

Production and characterization of amylase produced by Bacillus megaterium isolated from a local yam peel dumpsite in Minna, Niger State

Ten grams (10 g) of soil obtained from a local yam peel dumpsite in Minna, Niger State was analyzed. The bacterial isolates identified were Bacillus megaterium and Staphylococcus aureus.  Bacillus megaterium was used in the present study for the production of amylase enzyme as it recorded the largest zone of activity. Amylase activity was determined using DNS method. Highest yield of...

Author(s): S. B. Oyeleke, S. H. Auta and E. C. Egwim

October 2010

Novel pyrazolyl pyridazine derivatives likely to possess anti- inflammatory activity

Reaction of 4-acetyl-5,6-diphenylpyridazin-3(2H)-one (1) with diethyl oxalate in refluxing sodium ethoxide/ethanol mixture afforded ethyl 2,4-dioxo-4-(3-oxo-5,6-diphenyl-2,3-dihydropyridazin4-yl)butanoate (2) which with hydrazine hydrate (1:1 mol) in refluxing ethanol afforded ethyl5-(3-oxo-5,6-diphenyl-2,3-dihydropyridazin 4-yl)-1H-pyrazole-3-carboxylate (3) while reaction of 2 with hydrazine hydrate (1:2 moles) in...

Author(s): F. A. Yassin